Esteban-Amo María J, Telleria Amaia, Gobelli Dino, Serrano-Lorenzo Pablo, Tellería Juan J, Pérez-García María T, Kozlowski Piotr, de la Fuente Miguel Á, Simarro María
Department of Cell Biology, Genetics, Histology and Pharmacology, Faculty of Medicine, University of Valladolid, 47005, Valladolid, Spain.
Unit of Excellence Institute of Biomedicine and Molecular Genetics (IBGM), University of Valladolid and Spanish National Research Council (CSIC), 47003, Valladolid, Spain.
BMC Res Notes. 2025 Feb 25;18(1):83. doi: 10.1186/s13104-025-07149-8.
This study aimed to identify novel isoforms of mouse succinate dehydrogenase complex flavoprotein subunit A (Sdha) arising from internal exon skipping, analogous to the process observed in human ortholog SDHA.
We identified a novel isoform, designated Δ3-10, which lacked the final 104 nucleotides of exon 3 and all of exons 4 through 10, yet did not alter the reading frame. The Δ3-10 Sdha cDNA was cloned into expression vectors, and overexpression resulted in a protein localized to the mitochondria. However, the endogenous Δ3-10 Sdha protein was not detected with the available antibodies.
本研究旨在鉴定小鼠琥珀酸脱氢酶复合物黄素蛋白亚基A（Sdha）因内部外显子跳跃而产生的新型异构体，类似于在人类直系同源物SDHA中观察到的过程。
我们鉴定出一种新型异构体，命名为Δ3-10，它缺少外显子3的最后104个核苷酸以及外显子4至10的所有外显子，但未改变阅读框。将Δ3-10 Sdha cDNA克隆到表达载体中，过表达产生了一种定位于线粒体的蛋白质。然而，用现有的抗体未检测到内源性Δ3-10 Sdha蛋白。
