Barry Schroeder Amanda L, Reed Adam M, Radwan Osman, Bowen Loryn L, Ruiz Oscar N, Gunasekera Thusitha S, Hoffmann Andrea
Environmental Microbiology, Fuels & Combustion Division, University of Dayton Research Institute, Dayton, OH 45469, USA.
Power & Energy Division, University of Dayton Research Institute, Dayton, OH 45469, USA.
Biomolecules. 2025 Feb 4;15(2):227. doi: 10.3390/biom15020227.
Hydrocarbon fuel biofouling and biocorrosion require expensive cleanup of aviation infrastructures unless appropriate sustainment measures are applied. The identification of novel biological control agents offers promising alternatives to the current chemical biocides used in fuel sustainment. In this study, 496 microbial fuel isolates from our in-house repository were screened to identify new endogenously produced antimicrobial compounds. Using agar plug screening, liquid culture growth testing, and Jet A fuel culture assays, the two fuel-isolate strains #133, and #232 demonstrated promising biocontrol activity against bacteria, yeast, and filamentous fungi. Liquid chromatography-quadrupole time of flight tandem mass spectrometry (LC-QTOF-MS/MS) of #232 culture filtrate identified several common lipopeptide antimicrobials including gageostatin C, gageopeptin B, and miscellaneous macrolactins. In contrast, LC-QTOF-MS/MS identified the siderophore pyochelin as one of the predominant compounds in #133 culture filtrate with previously demonstrated antimicrobial effect. Jet fuel microbial consortium culture testing of #133 culture filtrate including flow-cytometry live/dead cell mechanism determination demonstrated antimicrobial action against Gram-positive bacteria. The study concludes that antimicrobial compounds secreted by #133 have bactericidal effects against sp. and cause cell death through bacterial lysis and membrane damage with potential applications in the biocidal treatment of hydrocarbon-based aviation fuels.
除非采取适当的维护措施，否则碳氢化合物燃料生物污垢和生物腐蚀需要对航空基础设施进行昂贵的清理。鉴定新型生物控制剂为目前用于燃料维护的化学生物杀灭剂提供了有前景的替代方案。在本研究中，对我们内部菌种库中的496株微生物燃料分离株进行了筛选，以鉴定新的内源性产生的抗菌化合物。通过琼脂块筛选、液体培养生长测试和喷气A燃料培养试验，两种燃料分离株#133和#232对细菌、酵母和丝状真菌表现出有前景的生物控制活性。#232培养滤液的液相色谱-四极杆飞行时间串联质谱（LC-QTOF-MS/MS）鉴定出几种常见的脂肽抗菌剂，包括抑芽菌素C、抑芽肽B和其他大环内酯类。相比之下，LC-QTOF-MS/MS鉴定出铁载体绿脓菌素是#133培养滤液中的主要化合物之一，此前已证明其具有抗菌作用。#133培养滤液的喷气燃料微生物群落培养测试，包括流式细胞仪活/死细胞机制测定，证明了对革兰氏阳性菌的抗菌作用。该研究得出结论，#133分泌的抗菌化合物对[具体菌种]具有杀菌作用，并通过细菌裂解和膜损伤导致细胞死亡，在基于碳氢化合物的航空燃料的生物杀灭处理中具有潜在应用。
