Lee Kyu-Il, Song Won-Seok, Han Seung-Kyu, Moon Kyung-Chul, Jeong Seong-Ho, Dhong Eun-Sang
Department of Plastic Surgery, Taean Public Health Office, Taean-gun 32148, Republic of Korea.
Department of Plastic Surgery, Korea University College of Medicine, Seoul 02841, Republic of Korea.
Bioengineering (Basel). 2025 Feb 3;12(2):145. doi: 10.3390/bioengineering12020145.
Our group has previously demonstrated that tissue-engineered dermis containing cultured fibroblasts or adipose-derived stromal vascular fraction cells is superior to artificial dermis in terms of scar quality for covering facial defects. However, using these cells for clinical applications requires Food and Drug Administration approval and involves complex procedures for cell culture or isolation. This retrospective study aimed to compare effects of tissue-engineered dermis containing micronized adipose tissue (MAT) and artificial dermis for facial reconstruction. Tissue-engineered dermis consisting of MAT seeded on artificial dermis was applied in 30 cases, while artificial dermis without MAT was grafted in 35 cases. Healing time and severities of scar contraction, color mismatch, and landmark distortion at one year after healing were evaluated. Wounds in the tissue-engineered dermis group re-epithelialized in 30.0 ± 4.3 days compared to 34.3 ± 5.4 days in the artificial dermis group ( < 0.05). The average dE2000 score in color mismatch analysis was 4.9 ± 1.7 in the tissue-engineered dermis group and 5.1 ± 1.7 in the artificial dermis group ( = 0.57). The extent of scar contraction was 16.2 ± 12.3% in the tissue-engineered dermis group and 23.2 ± 12.8% in the artificial dermis group ( < 0.05). The average severity grade of landmark distortion was 0.20 ± 0.50 in the tissue-engineered dermis group and 0.50 ± 0.71 in the artificial dermis group ( < 0.05). These findings indicate that tissue-engineered dermis grafts containing MAT are superior to artificial dermis grafts for facial reconstruction in terms of healing time, scar contraction, and landmark distortion severity. However, there was no significant difference in color mismatch between the two groups.
我们的研究小组之前已经证明，含有培养的成纤维细胞或脂肪来源的基质血管成分细胞的组织工程真皮在覆盖面部缺损的瘢痕质量方面优于人工真皮。然而，将这些细胞用于临床应用需要获得美国食品药品监督管理局的批准，并且涉及细胞培养或分离的复杂程序。这项回顾性研究旨在比较含有微化脂肪组织（MAT）的组织工程真皮和人工真皮对面部重建的效果。将接种有MAT的人工真皮组成的组织工程真皮应用于30例患者，而将不含MAT的人工真皮移植到35例患者中。评估愈合时间以及愈合后一年时瘢痕收缩、颜色不匹配和标志点变形的严重程度。组织工程真皮组伤口的再上皮化时间为30.0±4.3天，而人工真皮组为34.3±5.4天（P<0.05）。在颜色不匹配分析中，组织工程真皮组的平均dE2000评分为4.9±1.7，人工真皮组为5.1±1.7（P=0.57）。组织工程真皮组的瘢痕收缩程度为16.2±12.3%，人工真皮组为23.2±12.8%（P<0.05）。组织工程真皮组标志点变形的平均严重程度评分为0.20±0.50，人工真皮组为0.50±0.71（P<0.05）。这些结果表明，含有MAT的组织工程真皮移植物在愈合时间、瘢痕收缩和标志点变形严重程度方面优于人工真皮移植物用于面部重建。然而，两组之间在颜色不匹配方面没有显著差异。
