Zhang Jingqing, Xu Yikun, Wu Guize, Niu Chaoyong, Zhang Yibo, Hao Jinghong, Wang Baoju, Liu Ning
Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
National Engineering Research Center for Vegeta-bles, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Int J Mol Sci. 2025 Feb 16;26(4):1687. doi: 10.3390/ijms26041687.
The Aux/IAA proteins are key regulators of auxin signaling transduction, mediating various physiological and developmental processes in higher plants; however, little information on Aux/IAAs is known in lettuce, an economically important vegetable. In this study, a total of 29 genes were identified from the lettuce genome. Sequence alignment and domain analyses suggested the presence of conserved Aux/IAA subdomains (Domain I-IV) in those LsIAAs, and Phylogenetic analysis indicated that members of LsAux/IAA could be classified into 10 subgroups by their homology to Aux/IAAs, which is also supported by exon-intron structure, consensus motifs, and domain compositions. Transcriptome data suggested that most of the genes were expressed in all tissues, whereas some of them were preferentially expressed in specific tissues. Analysis of cis-acting elements indicated the genes might be regulated by hormonal treatments and abiotic stresses, which was confirmed by qRT-PCR experiments. Taken together, our study provides valuable information for further investigation of the biological roles of genes in high-temperature conditions.
Aux/IAA蛋白是生长素信号转导的关键调节因子，介导高等植物的各种生理和发育过程；然而，在经济上重要的蔬菜生菜中，关于Aux/IAA的信息知之甚少。在本研究中，从生菜基因组中总共鉴定出29个基因。序列比对和结构域分析表明，这些LsIAA中存在保守的Aux/IAA亚结构域（结构域I-IV），系统发育分析表明，LsAux/IAA成员可根据其与Aux/IAA的同源性分为10个亚组，外显子-内含子结构、共有基序和结构域组成也支持这一点。转录组数据表明，大多数基因在所有组织中都有表达，而其中一些基因在特定组织中优先表达。顺式作用元件分析表明，这些基因可能受激素处理和非生物胁迫的调控，qRT-PCR实验证实了这一点。综上所述，我们的研究为进一步研究这些基因在高温条件下的生物学作用提供了有价值的信息。
