Wu Zhengchang, Yu Luchen, Hu Yueqing, Bao Wenbin, Wu Shenglong
Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou 225009, China.
Vet Sci. 2025 Feb 2;12(2):117. doi: 10.3390/vetsci12020117.
Porcine circovirus type 2 (PCV2) is an important pathogen that leads to great economic losses to the swine industry. Paeoniflorin (PF), a novel plant extract, has been reported to have antiviral properties. However, the role of paeoniflorin in regulating PCV2 replication remains unclear. Here, we used the CCK8 assay to demonstrate that PF within safe concentrations (0-275 mM) significantly inhibits PCV2 replication in a dose-dependent manner in porcine kidney cells. Subsequently, comparative transcriptome and functional verification revealed that PF probably inherits PCV2 replication via targeting AKT/mTOR signaling. Further experimental data show that the AKT/mTOR signaling pathway is highly relevant to autophagy. Thus, experimental data from Western blot, qPCR, and the indirect immunofluorescence test indicate that PF inhibits PCV2 replication by inhibiting autophagy by targeting the AKT/mTOR signaling pathway. Together, our results provide insight into the mechanism of paeoniflorin in regulating PCV2 replication and offer new ideas for the treatment of PCV2 infection in pigs.
猪圆环病毒2型（PCV2）是一种重要的病原体，给养猪业造成了巨大的经济损失。芍药苷（PF）是一种新型植物提取物，据报道具有抗病毒特性。然而，芍药苷在调节PCV2复制中的作用仍不清楚。在此，我们使用CCK8试验证明，安全浓度（0-275 mM）的PF在猪肾细胞中以剂量依赖的方式显著抑制PCV2复制。随后，比较转录组和功能验证表明，PF可能通过靶向AKT/mTOR信号传导来抑制PCV2复制。进一步的实验数据表明，AKT/mTOR信号通路与自噬高度相关。因此，蛋白质免疫印迹、定量聚合酶链反应和间接免疫荧光试验的实验数据表明，PF通过靶向AKT/mTOR信号通路抑制自噬来抑制PCV2复制。总之，我们的研究结果为芍药苷调节PCV2复制的机制提供了见解，并为猪PCV2感染的治疗提供了新思路。
