Plant Biomass Allocation-Regulated Nitrogen and Phosphorus Addition Effects on Ecosystem Carbon Fluxes of a Lucerne ( ssp. ) Plantation in the Loess Plateau.

Nitrogen (N) and phosphorus (P) are key limiting factors for carbon (C) fluxes in artificial grasslands. The impact of their management on ecosystem C fluxes, including net ecosystem productivity (NEP), ecosystem respiration (ER), and gross ecosystem productivity (GEP) in the Loess Plateau is unclear. An experiment was conducted to study changes in these C fluxes with varying N (0, 5, 10, 15, and 20 g N m) and P (0 and 10 g P m) additions from 2022 to 2023 in a lucerne plantation. Results showed that N addition positively influenced NEP and GEP in the first year after planting with N addition at the rate of 10 g N m was optimal for C assimilation, but it had negligible effect on ER in both two years in the studied lucerne ( ssp. ) plantation. Phosphorus addition significantly increased ER and stimulated GEP, resulting in an increasing effect on NEP only at the early stage after planting. The addition of N and P enhanced soil N and P availability and further improved the leaf chemical stoichiometry characteristics, leading to changes in biomass distribution. The more belowground biomass under N addition and more aboveground production under P addition resulted in different responses of ecosystem C fluxes to N and P addition. The results suggest that the effects of N and P fertilization management on the ecosystem C cycle may be largely dependent on the distribution of above- and belowground plant biomass in the artificial grassland ecosystem.