Reisi Radin, Esfarjani Masih Sharifzadeh, Gandomkar Alireza, Mahsa Amirmohammad, Salehi Hossein, Reisi Parham, Jokar Zahra
Shahid Ejei 2 High School, Isfahan, Iran.
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2024 Dec 28;13:120. doi: 10.4103/abr.abr_449_23. eCollection 2024.
Burn as the most common injury disrupts the protective function of the skin and induces complications in patients. Therefore, the treatment of these patients presents a significant clinical challenge. This study evaluated the effects of () larvae and () earthworm extracts, alone or in combination, on the healing of third-degree burns in male mice.
A third-degree burn model was induced on the skin of the interscapular region. Then, the extracts of larvae and earthworms were topically applied separately or simultaneously every other day for a 21-day period. To evaluate the process of wound healing, macroscopic parameters were monitored and examined during the study period. Finally, the animals were sacrificed, and skin sampling was performed for histological investigations.
The results of the study showed that both extracts of larvae and earthworm accelerated the wound-healing process ( < 0.01). The group receiving extract of earthworm had better wound healing than the groups receiving Vaseline and silver sulfadiazine, and histological evidences confirmed these observations. However, the use of two extracts simultaneously did not affect the wound-healing process.
The results of this study demonstrated that the extracts of larvae and earthworm, especially , include effective compounds that can significantly enhance the rate of burn wound healing. However, more studies are needed to identify and purify the effective compounds of these extracts involved in the process of wound healing.
烧伤作为最常见的损伤,会破坏皮肤的保护功能并引发患者并发症。因此,对这些患者的治疗面临重大临床挑战。本研究评估了()幼虫提取物和()蚯蚓提取物单独或联合使用对雄性小鼠三度烧伤愈合的影响。
在肩胛间区域皮肤诱导建立三度烧伤模型。然后,每隔一天分别或同时局部应用幼虫和蚯蚓提取物,持续21天。为评估伤口愈合过程,在研究期间监测并检查宏观参数。最后,处死动物并进行皮肤取样以进行组织学研究。
研究结果表明,幼虫提取物和蚯蚓提取物均加速了伤口愈合过程(<0.01)。接受蚯蚓提取物的组比接受凡士林和磺胺嘧啶银的组伤口愈合更好,组织学证据证实了这些观察结果。然而,同时使用两种提取物并未影响伤口愈合过程。
本研究结果表明,幼虫提取物和蚯蚓提取物,尤其是(),含有可显著提高烧伤创面愈合速度的有效化合物。然而,需要更多研究来鉴定和纯化这些提取物中参与伤口愈合过程的有效化合物。