He Miao, Xie Wen-Qing, Cheng Guo, Li Wen-Ping, Yu Deng-Jie, Jin Hong-Fu, Deng Zhen-Han, Li Yu-Sheng
Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, China.
College of Veterinary Medicine, Hunan Agricultural University, Changsha, China.
Ann Palliat Med. 2021 Mar;10(3):2869-2879. doi: 10.21037/apm-20-2393. Epub 2021 Feb 23.
To investigated the effect of earthworm extract (EE) on deep second-degree burn wound healing process.
A burn wound model was created on the mice's skin and was subject to different treatments: the control group received no treatment; the Jingwanhong (JWH: a well-established, widely used external ointment for treating burn wounds) group was treated with 0.1 g of JWH cream and spray it on the wound surface; the EE group was treated with 0.1 mL of EE solution. All the mice were sacrificed at 3, 7, 11, and 15 days after injury (n=6/group/time point). Macroscopic observation, wound healing rate (WHR), wound healing time (WHT), water content (WC), hydroxyproline (Hyp) content, histological, and hematological analyses were performed at the burn wound sites.
Better, faster burn wound healing in the JWH and EE groups than the control group at 15 days after injury were detected at the wound sites. Compared to the control group, the EE group had higher WHR, shorter WHT, lower WC, higher Hyp content, more fibroblasts, fibrocytes, and capillary endothelial cells; in addition, they showed greater capillary endothelial cell grouping at the wound sites during the healing process. This group also showed more platelets, white blood cells (WBCs), and neutrophilic granulocytes in serum at the early stages after burn injury.
EE could effectively promote skin wound healing by decreasing edema, suppressing fibrosis, activating angiogenesis and epithelial regeneration, inhibiting scar formation, and reducing the risk of infection. Thus, it could be made into a promising healing agent for burn wound.
探讨蚯蚓提取物（EE）对深二度烧伤创面愈合过程的影响。
在小鼠皮肤上建立烧伤创面模型，并进行不同处理：对照组不做处理；京万红（JWH：一种成熟且广泛使用的治疗烧伤创面的外用软膏）组用0.1 g京万红乳膏涂抹并喷洒于创面；EE组用0.1 mL EE溶液处理。所有小鼠在受伤后3、7、11和15天处死（每组/每个时间点n = 6）。对烧伤创面部位进行宏观观察、创面愈合率（WHR）、创面愈合时间（WHT）、含水量（WC）、羟脯氨酸（Hyp）含量、组织学和血液学分析。
在伤后15天，观察到JWH组和EE组创面愈合情况优于对照组，愈合速度更快。与对照组相比，EE组WHR更高，WHT更短，WC更低，Hyp含量更高，成纤维细胞、纤维细胞和毛细血管内皮细胞更多；此外，在愈合过程中创面部位的毛细血管内皮细胞聚集更明显。该组在烧伤损伤后的早期血清中还显示出更多的血小板、白细胞（WBC）和中性粒细胞。
EE可通过减轻水肿、抑制纤维化、激活血管生成和上皮再生、抑制瘢痕形成以及降低感染风险来有效促进皮肤创面愈合。因此，它有望制成一种治疗烧伤创面的愈合剂。
