Bard H, Prosmanne J
Pediatrics. 1985 Jun;75(6):1143-7.
Erythrocytosis, extramedullary erythropoiesis, and increased levels of plasma erythropoietin have been observed in newborn infants of diabetic mothers. Because there is evidence that there is a relationship between increased fetal hemoglobin production and acute erythropoietic expansion, it was considered important to study the proportion of fetal hemoglobin and adult hemoglobin synthesis in newborn infants of insulin-dependent diabetic mothers. Samples from nine newborn infants of diabetic mothers as well as nine control infants, ranging from 36 to 38 weeks of gestation, were incubated in an amino acid mixture containing [14C]leucine. The adult hemoglobin and fetal hemoglobin were then separated by column chromatography on DEAE [O-(diethylaminoethyl)] Sephadex. To confirm that the fetal hemoglobin obtained after Sephadex chromatography was not contaminated with other hemoglobins, several of the DEAE separations from each group were reconstituted and subjected to polypeptide chain elution using carboxyl-methyl cellulose chromatography. The data demonstrated that the newborn infants of diabetic mothers are synthesizing significantly more fetal hemoglobin than is expected for their period of development (82.2 +/- 3.6 v 72.8 +/- 4.2; P less than .005). It is suggested that the in utero environment of the fetus of the diabetic mother causes an increase in fetal hemoglobin synthesis.
在糖尿病母亲的新生儿中观察到红细胞增多、髓外造血以及血浆促红细胞生成素水平升高。由于有证据表明胎儿血红蛋白产量增加与急性红细胞生成扩张之间存在关联，因此研究胰岛素依赖型糖尿病母亲的新生儿中胎儿血红蛋白和成人血红蛋白合成的比例被认为很重要。采集了9名糖尿病母亲的新生儿以及9名对照婴儿（孕周为36至38周）的样本，将其置于含有[14C]亮氨酸的氨基酸混合物中进行孵育。然后通过在DEAE [O-(二乙氨基乙基)] 葡聚糖上进行柱色谱分离成人血红蛋白和胎儿血红蛋白。为了确认葡聚糖色谱分离后获得的胎儿血红蛋白未被其他血红蛋白污染，对每组的几个DEAE分离物进行重组，并使用羧甲基纤维素色谱进行多肽链洗脱。数据表明，糖尿病母亲的新生儿合成的胎儿血红蛋白明显多于其发育阶段预期的量（82.2±3.6对72.8±4.2；P<0.005）。提示糖尿病母亲胎儿的宫内环境导致胎儿血红蛋白合成增加。
