Volk Marko, Šavli Dominik, Molan Katja, Terlep Saša, Levičnik-Höfferle Špela, Trost Mojca, Gašpirc Boris, Lukač Matjaž, Jezeršek Matija, Stopar David
University of Ljubljana, Department of Microbiology, Biotechnical Faculty, Ljubljana, Slovenia.
University of Ljubljana, Faculty of Mechanical Engineering, Ljubljana, Slovenia.
J Biomed Opt. 2025 Feb;30(2):025002. doi: 10.1117/1.JBO.30.2.025002. Epub 2025 Feb 25.
Here, we present a photoacoustic method to remove biofilms from periodontal and peri-implant-constrained geometries.
We aim to remove biofilms from narrow periodontal and peri-implant model systems with the application of Er:YAG ultrashort laser pulses.
Construction of zero-gap model system from PDMS and titanium, growth of biofilms on titanium surfaces, and removal of biofilms with Er:YAG USP, 20 mJ, 15 Hz, and 10 s were performed.
The results suggest that geometry, the vertical position of the laser fiber tip, and the evolution of the primary cavitation bubble significantly affect cleaning effectiveness. Cleaning was higher in the wedge part of the model system. In the zero-gap part of the model system, biofilm cleaning effectiveness was highest at the position of the laser fiber tip and decreased above and below the fiber tip. The dimension of the space in which the cavitation bubble develops determines the size and dynamics of the expanded cavitation bubble and consequently the biofilm cleaning effectiveness.
The obtained results suggest a very good biofilm removal effectiveness in difficult-to-reach narrow geometries mimicking clinical attachment loss in the periodontal/peri-implant pocket.
在此，我们提出一种光声方法，用于从牙周和种植体周围受限的几何结构中去除生物膜。
我们旨在通过应用铒钇铝石榴石超短激光脉冲，从狭窄的牙周和种植体周围模型系统中去除生物膜。
用聚二甲基硅氧烷（PDMS）和钛构建零间隙模型系统，在钛表面培养生物膜，并用能量为20 mJ、频率为15 Hz、持续时间为10 s的铒钇铝石榴石超短脉冲激光去除生物膜。
结果表明，几何形状、激光光纤尖端的垂直位置以及初生空化泡的演变显著影响清洁效果。在模型系统的楔形部分清洁效果更高。在模型系统的零间隙部分，生物膜清洁效果在激光光纤尖端位置最高，在光纤尖端上方和下方则降低。空化泡形成空间的尺寸决定了扩展空化泡的大小和动力学，从而决定了生物膜清洁效果。
所得结果表明，在模拟牙周/种植体周围袋临床附着丧失的难以触及的狭窄几何结构中，生物膜去除效果非常好。
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