Wang Yutong, Liu Yizhi, Liang Xiao, Liu Zhiyuan, Yang Yaling
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan Province, China.
Sinopharm Kunming Plasma-Derived Biotherapies Co., Ltd, Kunming, China.
Mikrochim Acta. 2025 Feb 27;192(3):193. doi: 10.1007/s00604-025-07051-x.
This study successfully developed a colorimetric/SERS dual-mode sensor for determining acrylamide in baked goods based on the excellent peroxidase-like activity and Raman activity of Fe-PHS@Au-Ag-Au Janus NPs. In colorimetric mode, the thiol-ene Michael addition between acrylamide (AA) and glutathione (GSH) efficiently eliminates GSH-induced peroxidase-like activity inhibition. The peroxidase-like activity of Fe-PHS@Au-Ag-Au Janus NPs gradually recovered, and the blue color of the solution gradually deepened with the increase in AA dosage. In surface-enhanced Raman spectroscopy (SERS), Apt-Fe-PHS@Au-Ag-Au Janus NPs can selectively capture AA and bind specifically, leading to the dissociation of Apt and Fe-PHS@Au-Ag-Au Janus NPs. The Raman activity of Apt-Fe-PHS@Au-Ag-Au Janus NPs decreases due to the dissociation of Apt. The dual-mode sensor was utilized for the determination of acrylamide in the concentrations range from 0.05 to 20 µg·L with detection limits of 0.06 µg·L (SERS) and 0.01 µg·L (colorimetric). The recovery in baked samples was between 91.0 and 108.0%.
本研究基于Fe-PHS@Au-Ag-Au Janus纳米粒子优异的过氧化物酶样活性和拉曼活性，成功开发了一种用于测定烘焙食品中丙烯酰胺的比色/表面增强拉曼散射（SERS）双模式传感器。在比色模式下，丙烯酰胺（AA）与谷胱甘肽（GSH）之间的硫醇-烯迈克尔加成反应有效消除了GSH诱导的过氧化物酶样活性抑制。Fe-PHS@Au-Ag-Au Janus纳米粒子的过氧化物酶样活性逐渐恢复，溶液的蓝色随着AA用量的增加而逐渐加深。在表面增强拉曼光谱（SERS）中，Apt-Fe-PHS@Au-Ag-Au Janus纳米粒子可以选择性地捕获AA并特异性结合，导致Apt与Fe-PHS@Au-Ag-Au Janus纳米粒子解离。由于Apt的解离，Apt-Fe-PHS@Au-Ag-Au Janus纳米粒子的拉曼活性降低。该双模式传感器用于测定浓度范围为0.05至20μg·L的丙烯酰胺，检测限为0.06μg·L（SERS）和0.01μg·L（比色法）。烘焙样品中的回收率在91.0%至108.0%之间。
