Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, International Joint Research Center for Photoresponsive Molecules and Materials, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China.
College of Science and Technology, Hebei Agricultural University, Cangzhou, Hebei 061100, China.
Food Chem. 2023 Jun 30;412:135526. doi: 10.1016/j.foodchem.2023.135526. Epub 2023 Jan 20.
Ochratoxin A (OTA) was a dangerous biological toxin, and would easily contaminate food and induced food safety problems. The development of electrochemical aptasensors by designing strong and anti-interfere electroactive labels could improve the sensitivity and accuracy of OTA detection. In this contribution, novel electroactive Au-AgPd Janus NPs were firstly synthesized and exhibited electroreduction signal at -0.4 V, owing to the reduction process of Pd. The electroreduction signal was amplified 1.5 times under local surface plasmon resonance (LSPR) excitation, which could improve the sensitivity of OTA detection. Plasmon-enhanced electroreduction principle of Au-AgPd Janus NPs was verified, which endowed electrochemical aptasensor with high accuracy and anti-interference ability for OTA detection. Au-AgPd Janus NPs served as electrochemical beacon achieved sensitive and accurate OTA detection with the limit of detection (LOD) of 0.98 pM. This work opens up new directions for the construction of electroactive heterostructures for the sensitive and accurate biotoxins electroanalytical applications.
赭曲霉毒素 A（OTA）是一种危险的生物毒素，容易污染食物并引发食品安全问题。通过设计强抗干扰电活性标记物来开发电化学生物传感器，可以提高 OTA 检测的灵敏度和准确性。在本研究中，首次合成了新型的电活性 Au-AgPd 双金属纳米粒子，并在-0.4 V 处表现出电还原信号，这归因于 Pd 的还原过程。在局域表面等离子体共振（LSPR）激发下，电还原信号放大了 1.5 倍，从而提高了 OTA 检测的灵敏度。验证了 Au-AgPd 双金属纳米粒子的等离子体增强电还原原理，为电化学生物传感器提供了高准确性和抗干扰能力，用于 OTA 的检测。Au-AgPd 双金属纳米粒子作为电化学生物探针，实现了对 OTA 的灵敏和准确检测，检测限（LOD）为 0.98 pM。这项工作为构建用于灵敏和准确生物毒素电化学分析应用的电活性异质结构开辟了新的方向。
