Cai Shenwen, Zhao Jun, Sheng Enguo, Fan Leilei, Shen Ziwei, Li Yunfeng
College of Resources and Environment, Zunyi Normal University, Zunyi, 563006, China.
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China.
Sci Rep. 2025 Feb 26;15(1):6974. doi: 10.1038/s41598-025-91664-y.
Bacteria and micro-eukaryotes play important roles in river ecological systems. The processes that govern bacterial and micro-eukaryotic communities in urban rivers are still uncertain. The spatiotemporal characteristics and assembly processes of bacterial and micro-eukaryotic communities in the Xiangjianghe River were explored using 16 S and 18 S rRNA gene amplicon sequencing in the present study. The results indicate that the bacterial and micro-eukaryotic community composition exhibited distinct temporal and spatial variation. The topological characteristics of co-occurrence networks demonstrate that the bacterial and micro-eukaryotic community coexistence patterns vary significantly between the four seasons. Water temperature (WT) and oxidation-reduction potential (ORP) were detected as the most critical factors affecting bacterial and micro-eukaryotic community structure. The stochastic process (dispersal limitation) was the dominant assembly process for bacteria and micro-eukaryotes in all seasons. Deterministic and stochastic processes influenced the bacteria and micro-eukaryotes differently. Compared to bacteria, the values of niche breadth were relatively lower, and the proportion of deterministic processes was relatively higher in micro-eukaryotes. These results expand our understanding of spatiotemporal patterns, assembly mechanisms, and influencing factors of bacterial and micro-eukaryotic communities in urban rivers.
细菌和微型真核生物在河流生态系统中发挥着重要作用。城市河流中控制细菌和微型真核生物群落的过程仍不明确。本研究利用16S和18S rRNA基因扩增子测序技术，探究了湘江细菌和微型真核生物群落的时空特征及组装过程。结果表明，细菌和微型真核生物群落组成呈现出明显的时空变化。共现网络的拓扑特征表明，细菌和微型真核生物群落的共存模式在四季之间存在显著差异。水温（WT）和氧化还原电位（ORP）被检测为影响细菌和微型真核生物群落结构的最关键因素。随机过程（扩散限制）是所有季节中细菌和微型真核生物的主要组装过程。确定性和随机过程对细菌和微型真核生物的影响不同。与细菌相比，微型真核生物的生态位宽度值相对较低，确定性过程的比例相对较高。这些结果扩展了我们对城市河流中细菌和微型真核生物群落时空模式、组装机制及影响因素的理解。
