State Key Laboratory of Marine Environmental Science, Key Laboratory of the Ministry of Education for Coastal Wetland Ecosystems, College of the Environment and Ecology, Xiang'an South Road, Xiamen 361102, China.
Fujian Xiamen Environmental Monitoring Central Station, Xing'lin South Road, Xiamen 361022, China.
Sci Total Environ. 2024 Jan 1;906:167499. doi: 10.1016/j.scitotenv.2023.167499. Epub 2023 Sep 30.
Systematic studies on the assembly process and driving mechanisms of microbial communities in estuaries with diverse seasonal and spatial scales are still limited. In this study, high-throughput sequencing, and microbial network analysis were combined to decipher the impact of environmental changes and biological interactions on the maintenance of microbial diversity patterns in the Jiulong River Estuary (JRE). The results showed that overall, stochastic processes dominated the bacterioplankton community assembly in the estuary, accounting for 49.66-74.78 % of the total. Additionally, bacterioplankton community diversity varied significantly across seasons and subzones. Specifically, the concentration of soluble reactive phosphorus (SRP) in the estuary steadily reduced from winter to summer, and the corresponding bacterioplankton community interactions gradually shifted from the weakest interaction in winter to the strongest in summer. The deterministic processes contributed more than half (50.34 %) to microbial assembly in the summer, but only 25.22 % in winter. Deterministic processes prevailed in the seaward with low SRP concentrations and strong bacterioplankton community interactions, while stochastic processes contributed 70.14 % to the assembly of microbial communities riverward. Biotic and abiotic factors, such as nutrients and microbial interactions, jointly drove the seasonal and spatial patterns of bacterioplankton community assembly, but overall, nutrients played a dominant role. Nevertheless, the contributions of nutrients and microbial interactions were equivalent in spatial assembly processes, albeit nutrients were the primary seasonal driver of the bacterioplankton community assembly process. This study emphasizes the significance of microbial interactions in the bacterioplankton community assemblage. These findings provide new and comprehensive insights into the microbial communities' organization in estuaries.
系统研究不同季节和空间尺度河口微生物群落的组装过程和驱动机制仍然有限。本研究结合高通量测序和微生物网络分析，解析环境变化和生物相互作用对九龙江河口（JRE）微生物多样性模式维持的影响。结果表明，总体而言，随机过程主导了河口浮游细菌群落的组装，占总组装的 49.66-74.78%。此外，浮游细菌群落多样性在季节和亚区之间存在显著差异。具体而言，河口可溶性反应磷（SRP）浓度从冬季到夏季稳步降低，相应的浮游细菌群落相互作用逐渐从冬季的最弱相互作用转变为夏季的最强相互作用。确定性过程对夏季微生物组装的贡献超过一半（50.34%），但在冬季仅为 25.22%。确定性过程在 SRP 浓度低且浮游细菌群落相互作用强的向海方向占主导地位，而随机过程对河流方向微生物群落组装的贡献为 70.14%。生物和非生物因素，如营养物质和微生物相互作用，共同驱动浮游细菌群落组装的季节性和空间模式，但总体而言，营养物质起主导作用。然而，在空间组装过程中，营养物质和微生物相互作用的贡献相当，尽管营养物质是浮游细菌群落组装过程的主要季节性驱动因素。本研究强调了微生物相互作用在浮游细菌群落组装中的重要性。这些发现为河口微生物群落的组织提供了新的和全面的见解。
