Miao Pei, Wang Haijun, Wang Wei, Wang Zhengdong, Ke Han, Cheng Hangyuan, Ni Jinjing, Liang Jingnan, Yao Yu-Feng, Wang Jizong, Zhou Jian-Min, Lei Xiaoguang
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
Science. 2025 Feb 28;387(6737):eads0377. doi: 10.1126/science.ads0377.
Numerous gram-negative bacterial pathogens employ the type III secretion system (T3SS), a multiprotein injectisome, to deliver virulence proteins into host cells and cause diseases. We uncover erucamide as a previously unknown phytoalexin of both dicots and monocots that blocks the T3SS function of multiple bacterial pathogens. Genetically impairing erucamide accumulation or exogenous application in highlighted erucamide's role in antibacterial immunity. Erucamide binds Hypersensitive response and conserved C (HrcC), a key T3SS component, to block injectisome assembly. Analyses of erucamide analogs and HrcC mutants indicated that the erucamide-HrcC binding is required for inhibiting T3SS in vitro and antibacterial resistance in plants, suggesting an essential role of erucamide-HrcC binding in disease resistance. This work reveals a plant chemical defense that targets major virulence machinery in bacterial pathogens.
许多革兰氏阴性细菌病原体利用III型分泌系统（T3SS），一种多蛋白注射体，将毒力蛋白输送到宿主细胞中并引发疾病。我们发现芥酸酰胺是一种此前未知的双子叶植物和单子叶植物的植物抗毒素，它能阻断多种细菌病原体的T3SS功能。遗传上损害芥酸酰胺的积累或在……中进行外源应用突出了芥酸酰胺在抗菌免疫中的作用。芥酸酰胺与超敏反应和保守C（HrcC）结合，HrcC是T3SS的关键组分，以阻断注射体组装。对芥酸酰胺类似物和HrcC突变体的分析表明，芥酸酰胺与HrcC的结合是体外抑制T3SS和植物抗菌抗性所必需的，这表明芥酸酰胺与HrcC的结合在抗病性中起着至关重要的作用。这项工作揭示了一种针对细菌病原体主要毒力机制的植物化学防御。
