Watkinson Rebecca L, Bochkov Yury A, Gern James E, Iosifidis Thomas, Looi Kevin, Laing Ingrid A, Kicic Anthony
Division of Paediatrics, School of Medicine, The University of Western Australia, Crawley, WA, Australia.
Wal-Yan Respiratory Research Centre, The Kids Research Institute Australia, Nedlands, WA, Australia.
Methods Mol Biol. 2025;2903:113-139. doi: 10.1007/978-1-0716-4410-2_10.
The nasal epithelium is the primary point of contact for inhaled respiratory viruses such as rhinovirus, respiratory syncytial virus, influenza, and coronavirus, among others. In order to establish infection, these viruses must engage their respective receptors located on host epithelial cells and begin replication. However, the nasal epithelium is also a pivotal orchestrator of both structural and innate immune defenses against these pathogens and thus mounts a broad antiviral response to halt the progression of the infection into the lower airways. Of note, the most common virus found in the airways of children presenting to the hospital emergency department with acute wheezing and asthma is rhinovirus C (RV-C), followed by rhinovirus A (RV-A). Here, we illustrate infection of a preclinical differentiated nasal epithelial model with clinical isolates of RV-A and -C, in conjunction with several methods utilized for characterization of epithelial responses post-infection in vitro.
鼻上皮是鼻病毒、呼吸道合胞病毒、流感病毒和冠状病毒等吸入性呼吸道病毒的主要接触点。为了建立感染，这些病毒必须与宿主上皮细胞上各自的受体结合并开始复制。然而，鼻上皮也是针对这些病原体的结构和固有免疫防御的关键协调者，因此会产生广泛的抗病毒反应，以阻止感染向下呼吸道发展。值得注意的是，在因急性喘息和哮喘到医院急诊科就诊的儿童气道中发现的最常见病毒是鼻病毒C（RV-C），其次是鼻病毒A（RV-A）。在这里，我们展示了用RV-A和-C的临床分离株感染临床前分化的鼻上皮模型，并结合了几种用于体外表征感染后上皮反应的方法。
