低浓度次氯酸对鼻上皮细胞感染鼻病毒的影响。
The effect of a low concentration of hypochlorous acid on rhinovirus infection of nasal epithelial cells.
机构信息
Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
出版信息
Am J Rhinol Allergy. 2011 Jan-Feb;25(1):40-4. doi: 10.2500/ajra.2011.25.3545.
BACKGROUND
Low concentrations of hypochlorous acid (HOCl) have been shown to exhibit both antibacterial and anti-influenza virus activity, but HOCl still has not been used to kill human rhinovirus (HRV). To model the antiviral effect of nasal irrigation with low-level HOCl in patients with the common cold, we tested the effects of a low concentration of HOCl on HRV infection of primary human nasal epithelial cells (HNEC).
METHODS
Cells were infected with HRV for 24 hours and treated with HOCl three times, for 5 minutes each time, at 12 hour intervals. The effects of HOCl on rhinovirus-induced secretion of IL-6 and IL-8 were assessed by ELISA and HRV replication was determined by viral titration.
RESULTS
HOCl treatment significantly inhibited HRV-induced secretion of IL-6 and IL-8 and significantly reduced viral titer. The effects of HOCl peaked at 1 minute after HOCl generation and decreased thereafter.
CONCLUSION
These in vitro findings indicate that nasal irrigation with low-level HOCl solution may improve clinical symptoms in patients with the common cold.
背景
低浓度次氯酸（HOCl）已被证明具有抗菌和抗流感病毒活性，但 HOCl 仍未被用于杀死人类鼻病毒（HRV）。为了模拟低水平 HOCl 鼻腔冲洗对普通感冒患者的抗病毒作用，我们测试了低浓度 HOCl 对原代人鼻上皮细胞（HNEC）中 HRV 感染的影响。
方法
细胞用 HRV 感染 24 小时，然后每隔 12 小时用 HOCl 处理 3 次，每次 5 分钟。通过 ELISA 评估 HOCl 对鼻病毒诱导的 IL-6 和 IL-8 分泌的影响，并通过病毒滴定法确定 HRV 复制。
结果
HOCl 处理显著抑制 HRV 诱导的 IL-6 和 IL-8 的分泌，并显著降低病毒滴度。HOCl 的作用在 HOCl 生成后 1 分钟达到峰值，此后降低。
结论
这些体外发现表明，用低水平的 HOCl 溶液冲洗鼻腔可能会改善普通感冒患者的临床症状。
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