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草酸钙肾结石微环境特征识别中多组学的自我控制研究

Self-control study of multi-omics in identification of microenvironment characteristics in calcium oxalate kidney stones.

作者信息

Xu Shang, Liu Zhi-Long, Zhang Tian-Wei, Li Bin, Cao Yuan-Chao, Wang Xin-Ning, Jiao Wei

机构信息

Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266000, China.

出版信息

BMC Nephrol. 2025 Feb 27;26(1):104. doi: 10.1186/s12882-025-04026-1.

DOI:10.1186/s12882-025-04026-1
PMID:40016672
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11869433/
Abstract

BACKGROUND

Perform proteomic and metabolomic analysis on bilateral renal pelvis urine of patients with unilateral calcium oxalate kidney stones to identify the specific urinary microenvironment associated with stone formation.

METHODS

Using cystoscopy-guided insertion of ureteral catheters, bilateral renal pelvis urine samples are collected. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is employed to identify differential proteins and metabolites in the urine microenvironment. Differentially expressed proteins and differential metabolites are further analyzed for their biological functions and potential metabolic pathways through Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, Reactome pathway analysis and Biomolecular Interaction Network Database protein-protein interaction (PPI) network analysis.

RESULTS

In the urine from the stone-affected side, 36 differential proteins were significantly upregulated, 4 differential proteins were downregulated, and 10 differential metabolites were significantly upregulated. Functional and pathway analyses indicate that the differentially expressed proteins are primarily involved in inflammatory pathways and complement and coagulation cascades, while the differential metabolites are mainly associated with oxidative stress.

CONCLUSION

The proteomic and metabolomic profiles of the urinary microenvironment in stone-affected kidneys provide a more precise reflection of the pathophysiological mechanisms involved in stone formation and development.

摘要

背景

对单侧草酸钙肾结石患者的双侧肾盂尿液进行蛋白质组学和代谢组学分析，以确定与结石形成相关的特定尿液微环境。

方法

采用膀胱镜引导下输尿管导管插入术收集双侧肾盂尿液样本。运用液相色谱-串联质谱法（LC-MS/MS）鉴定尿液微环境中的差异蛋白质和代谢物。通过基因本体论（GO）分析、京都基因与基因组百科全书（KEGG）富集分析、Reactome通路分析以及生物分子相互作用网络数据库蛋白质-蛋白质相互作用（PPI）网络分析，进一步分析差异表达蛋白质和差异代谢物的生物学功能及潜在代谢途径。

结果

在患侧尿液中，36种差异蛋白质显著上调，4种差异蛋白质下调，10种差异代谢物显著上调。功能和通路分析表明，差异表达蛋白质主要参与炎症途径以及补体和凝血级联反应，而差异代谢物主要与氧化应激相关。

结论

患侧肾脏尿液微环境的蛋白质组学和代谢组学特征更精确地反映了结石形成和发展所涉及的病理生理机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fd4/11869433/2c3a616ebabb/12882_2025_4026_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fd4/11869433/4cfb460ee4fb/12882_2025_4026_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fd4/11869433/2c3a616ebabb/12882_2025_4026_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fd4/11869433/4cfb460ee4fb/12882_2025_4026_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fd4/11869433/120ca138ede5/12882_2025_4026_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fd4/11869433/eed3f86009a4/12882_2025_4026_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fd4/11869433/5c5f5c724ee0/12882_2025_4026_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fd4/11869433/967e28797bb7/12882_2025_4026_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fd4/11869433/e0ea1f1142d1/12882_2025_4026_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fd4/11869433/2c3a616ebabb/12882_2025_4026_Fig7_HTML.jpg

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