Chen Jialong, Xue Wenchang, Yao Zhanxin, Wang Chao, Zhu Wanjun, Wang He, Zhang Jipei, Tang Yi, Liu Rongchang, Chen Jidang
School of Animal Science and Technology, Foshan University, Foshan, China.
WANMUZHOU Biotechnology Limited Company, Foshan, China.
Front Vet Sci. 2025 Feb 13;12:1521705. doi: 10.3389/fvets.2025.1521705. eCollection 2025.
Goose circovirus (GoCV) is a recently identified pathogen in geese that is known to cause slow growth, feather disorder syndrome, and immunosuppression. Infection with GoCV may increase the risk of coinfections with multiple pathogens, leading to significant economic losses in the goose industry. However, due to a lack of serological detection methods, analysis of viral nucleic acids has been widely used in GoCV epidemiological surveys, which has limited accurate monitoring of the prevalence of GoCV. In this study, we developed and optimized an indirect ELISA method based on the prokaryotic-expressed recombinant GoCV capsid protein (△Cap-iELISA). The △Cap-iELISA was then used to test 349 goose serum samples collected from Guangdong, Shandong, and Fujian provinces during 2023 and 2024. The results showed that the positive rate of GoCV antibodies in the sampled geese was 71.06%. Further analysis indicated that the positive rate of GoCV antibodies increased with the age of the geese. In conclusion, we have developed a novel iELISA method that is well-suited for large-scale clinical detection and early diagnosis of GoCV infection. Notably, a significant correlation between age and the positive rate of GoCV antibodies among geese was observed based on this newly established method.
鹅圆环病毒（GoCV）是一种最近在鹅中发现的病原体，已知会导致生长缓慢、羽毛紊乱综合征和免疫抑制。感染GoCV可能会增加与多种病原体合并感染的风险，给养鹅业造成重大经济损失。然而，由于缺乏血清学检测方法，病毒核酸分析已广泛应用于GoCV的流行病学调查，这限制了对GoCV流行情况的准确监测。在本研究中，我们开发并优化了一种基于原核表达的重组GoCV衣壳蛋白的间接ELISA方法（△Cap-iELISA）。然后使用△Cap-iELISA检测了2023年至2024年期间从广东、山东和福建三省采集的349份鹅血清样本。结果显示，采样鹅中GoCV抗体的阳性率为71.06%。进一步分析表明，GoCV抗体的阳性率随鹅龄增加而升高。总之，我们开发了一种新型的iELISA方法，非常适合GoCV感染的大规模临床检测和早期诊断。值得注意的是，基于这种新建立的方法，观察到鹅的年龄与GoCV抗体阳性率之间存在显著相关性。
