Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Da Dian Biotechnology Company Limited, Pingtung, Taiwan.
J Vet Sci. 2021 Jan;22(1):e1. doi: 10.4142/jvs.2021.22.e1.
Goslings in several Taiwanese farms experienced gosling feather loss disease (GFL) at 21-35 days and goose broke feather disease (GBF) at 42-60 days. The prevalence ranges from a few birds to 500 cases per field. It is estimated that about 12,000 geese have been infected, the morbidity is 70-80% and the mortality is 20-30%.
This study aims to investigate the pathogens that cause GFL and GBF. Focus on the study of the correlation between goose circovirus (GoCV) and goose parvovirus (GPV) with the goose feather loss in southern Taiwan. Furthermore, a phylogenetic tree was established to align the differences between southern and northern Taiwan and compare with virus strains from China and Europe.
Samples were collected from animal hospitals. Molecular and microscopy diagnostics were used to examine 92 geese. Specific quantitative polymerase chain reaction (Q-PCR) assays are performed to evaluate GPV and GoCV viral loads and simultaneously evaluated the feather loss conditions in geese with the scoring method.
High prevalence of GoCV and GPV infection in geese showing signs of GFL and GBF. Inclusion body was detected in the feather follicles and Lieberkühn crypt epithelial cells. The Q-PCR showed the high correlation between feather loss and viruses during 3rd-5th week. However, the infection was not detected using the same test in 60 healthy geese.
Thus, GFL and GBF appear to be significantly closely related to GoCV and GPV. The geese feathers showed increasing recovery after being quarantined and disinfected.
台湾多家农场的雏鹅在 21-35 日龄时出现鹅羽毛损失病（GFL），42-60 日龄时出现鹅断羽病（GBF）。流行范围从几只禽鸟到每个农场 500 例不等。估计约有 12000 只鹅受到感染，发病率为 70-80%，死亡率为 20-30%。
本研究旨在调查引起 GFL 和 GBF 的病原体。重点研究鹅圆环病毒（GoCV）和鹅细小病毒（GPV）与台湾南部鹅羽毛损失的相关性。此外，建立了一个系统发育树来比较台湾南部和北部之间的差异，并与中国和欧洲的病毒株进行比较。
从动物医院采集样本。使用分子和显微镜诊断检查了 92 只鹅。进行特定的定量聚合酶链反应（Q-PCR）检测以评估 GPV 和 GoCV 的病毒载量，并同时使用评分法评估鹅的羽毛损失情况。
在出现 GFL 和 GBF 症状的鹅中，GoCV 和 GPV 的感染率很高。在羽毛滤泡和Lieberkühn 隐窝上皮细胞中检测到包涵体。Q-PCR 显示羽毛损失与病毒之间存在高度相关性，发生在第 3-5 周。然而，在 60 只健康鹅中使用相同的检测方法并未检测到感染。
因此，GFL 和 GBF 似乎与 GoCV 和 GPV 密切相关。被隔离和消毒后，鹅的羽毛显示出逐渐恢复。
