Pál Magda, Rahman Altafur, Hamow Kamirán Áron, Nagy Katalin, Janda Tibor, Dernovics Mihály, Szalai Gabriella
Hungarian Research Network, Centre for Agricultural Research, Agricultural Institute, Martonvásár, Brunszvik 2, H-2462, Hungary.
Plant Physiol Biochem. 2025 May;222:109659. doi: 10.1016/j.plaphy.2025.109659. Epub 2025 Feb 18.
Information about genotype- or light-dependent differences in the dynamics of polyamine uptake and metabolism is limited in plants. In the present study, these differences were characterised in putrescine-treated three wheat genotypes grown on hydroponic solution in growth chamber. Putrescine treatment induced putrescine accumulation especially in the roots; however, distinct variations indicated different regulation strategies among the genotypes. In Mv Béres, decreased expression of polyamine synthesis and back-conversion-related genes was detected, which partly mitigated excessive putrescine build-up, reaching a 976% increase maximum after 13 h, compared to the 0-h-control. In Mv Emese, the putrescine synthesis and absorption decreased, the synthesis and back-conversion of higher polyamines were not, but the terminal catabolism was initiated, and after 5 h and 642% maximum increase resulted in successful catabolism of putrescine excess. In TC33 a lower, but persistent putrescine accumulation was found with 412, 359, 418 and 419% increases after 1, 3, 5 and 7 h, respectively. However, the illumination had less influence on the root polyamine-pools than the exogenous polyamine treatments. The effects of putrescine or spermidine treatments on polyamine homeostasis were also monitored under continuous light or dark in Mv Emese. In contrast to the roots, at least in a 24-h period, the circadian rhythm had a primary influence, rather than polyamine treatments or the absence of light in the leaves. Understanding how the genotype and light influence polyamine metabolism contributes to selecting genotypes with favourable polyamine regulatory mechanisms and optimising crop management strategies, leading to improved crop productivity.
关于植物中多胺吸收和代谢动态的基因型或光依赖性差异的信息有限。在本研究中，对在生长室水培溶液中生长的三种经腐胺处理的小麦基因型的这些差异进行了表征。腐胺处理诱导了腐胺的积累，尤其是在根部；然而，明显的差异表明不同基因型之间存在不同的调控策略。在Mv Béres中，检测到多胺合成和逆转化相关基因的表达降低，这部分缓解了过量腐胺的积累，与0小时对照相比，13小时后最大增加了976%。在Mv Emese中，腐胺的合成和吸收减少，高级多胺的合成和逆转化未受影响，但启动了末端分解代谢，5小时后最大增加642%，成功分解了过量的腐胺。在TC33中，发现腐胺积累较低但持续，分别在1、3、5和7小时后增加了412%、359%、418%和419%。然而，光照对根部多胺池的影响小于外源多胺处理。在Mv Emese中，还在连续光照或黑暗条件下监测了腐胺或亚精胺处理对多胺稳态的影响。与根部相反，至少在24小时内，昼夜节律在叶片中起主要作用，而不是多胺处理或光照缺失。了解基因型和光照如何影响多胺代谢有助于选择具有有利多胺调控机制的基因型并优化作物管理策略，从而提高作物生产力。
