Majláth Imre, Benczúr Kinga, Rahman Altafur, Janda Tibor, Likó István, Kádas János, Pál Magda
Hungarian Research Network, Centre for Agricultural Research, Agricultural Institute, Brunszvik 2, Martonvásár, 2462, Hungary.
Department of Plant Physiology and Ecology, Institute of Agronomy, MATE, Villányi 29-43, Budapest, 1118, Hungary.
Sci Rep. 2025 Jul 2;15(1):22734. doi: 10.1038/s41598-025-08184-y.
Polyamines play a pivotal role in plant growth and development by modulating primary metabolism and gene expression patterns. However, their regulatory effect on epigenetics is still not clear. The impact of exogenous putrescine in wheat leaves was investigated to reveal novel insights into the intricate relationship between polyamines and DNA methylation. Since the outcome of putrescine treatment may vary depending on the light quality, the experiment was performed under both white and blue light, and MeDIP-seq method was applied to reveal the global methylation profile. Metabolite profiling revealed distinct changes induced by blue light and/or putrescine, and that the effect of putrescine in most cases was dominant under blue light. Blue light also exerted strong effects on methylation, and putrescine application could slightly induce further changes. Putrescine had a higher impact under white light, namely parallel with increased leaf spermidine level, a lower level of up-methylated genes involved in cellular component, but a higher level of down-methylated genes involved in molecular function and biological processes occurred compared to the blue light. Our results demonstrated that putrescine excess has a regulatory role in DNA methylation. These findings are also useful in understanding the relationship between polyamine metabolism and light conditions.
多胺通过调节初级代谢和基因表达模式在植物生长发育中发挥关键作用。然而，它们对表观遗传学的调节作用仍不清楚。研究了外源腐胺对小麦叶片的影响，以揭示多胺与DNA甲基化之间复杂关系的新见解。由于腐胺处理的结果可能因光质而异，因此在白光和蓝光下均进行了实验，并应用甲基化DNA免疫沉淀测序（MeDIP-seq）方法来揭示全基因组甲基化图谱。代谢物谱分析揭示了蓝光和/或腐胺诱导的明显变化，并且在大多数情况下，腐胺在蓝光下的作用占主导地位。蓝光也对甲基化有强烈影响，施用腐胺可轻微诱导进一步变化。与蓝光相比，腐胺在白光下具有更高的影响，即与叶片亚精胺水平升高同时，参与细胞成分的上调甲基化基因水平较低，但参与分子功能和生物学过程的下调甲基化基因水平较高。我们的结果表明，过量的腐胺在DNA甲基化中具有调节作用。这些发现对于理解多胺代谢与光照条件之间的关系也很有用。
