[Endotoxin recognition with myelopoiesis of monocytes and macrophages].

Cells from the monocyte-macrophage pathway release colony-stimulating factors. The release of colony-stimulating activity is increased upon endotoxin activation in the presence of fresh serum. Heat-inactivation of serum (56 degrees C, 30 minutes) abolishes the endotoxin-mediated activation of macrophages. Decomplementation with zymosan, iodipamide and the use of commercially serum deficient in C3 provide indirect evidence for a complement dependence of the endotoxin-macrophage interaction, which leads to release of colony-stimulating activity.