Kubota K, Motoyoshi K, Kajigaya S, Suda T, Saito M, Miura Y
Exp Hematol. 1983 May;11(5):364-70.
Murine bone marrow granulocyte/macrophage progenitor cells proliferated to form colonies consisting of their progeny in serum-free agar cultures in the presence of colony-stimulating activity. The cloning efficiency for the serum-free cultures was 80% of that for control cultures containing 20% fetal calf serum. Examinations of the cellular compositions of individual colonies revealed that the number of pure macrophage colonies was indistinguishable in the two culture systems while that of pure granulocyte and granulocyte/macrophage mixed colonies was smaller in the serum-free cultures.
在集落刺激活性存在的情况下，小鼠骨髓粒细胞/巨噬细胞祖细胞在无血清琼脂培养物中增殖，形成由其后代组成的集落。无血清培养物的克隆效率是含有20%胎牛血清的对照培养物的80%。对单个集落的细胞组成进行检查发现，在两种培养系统中，纯巨噬细胞集落的数量没有差异，而在无血清培养物中，纯粒细胞和粒细胞/巨噬细胞混合集落的数量较少。
