Sedtananun Saranya, Sujiwattanarat Penporn, Hoang Tra My Ha, Suphakun Prapasri, Seetaha Supaphorn, Auetragul Anon, Choowongkomon Kiattawee, Tabtimmai Lueacha
Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of b Technology North Bangkok, Bangkok 10800, Thailand.
Food and Agro-Industrial Research Center, King Mongkut's University of Technology North Bangkok, Bangkok 10800,Thailand.
Asian Pac J Cancer Prev. 2025 Feb 1;26(2):479-487. doi: 10.31557/APJCP.2025.26.2.479.
To evaluate the anti-inflammatory and anticancer effects of Phallus indusiatus extracts, particularly in modulating inflammatory microenvironments and sensitizing lapatinib-induced cytotoxicity in breast cancer cells.
Phallus indusiatus (bamboo mushroom) extracts were prepared using water extraction from fresh and dried specimens. The anti-inflammatory effects were assessed using RAW246.7 cells by measuring NO production, while cytotoxicity and proliferation effects were evaluated in MCF-7 and MCF-10A breast cell lines. To mimic the inflammatory microenvironment of breast cancer, MCF-7 cells were treated with TNFα to induce lapatinib resistance. The effects of the extracts on cell viability, apoptosis, and NF-kappaB signaling were evaluated under inflammatory microenvironment-mimicking conditions. Co-treatment with lapatinib and the extracts was analyzed for synergistic cytotoxic effects and pathway modulation.
P. indusiatus extracts demonstrated anti-inflammatory effects by reducing NO production in RAW246.7 cells. The extracts showed no cytotoxicity on MCF-7 and MCF-10A cells, with an observed proliferation increase in MCF-10A but not MCF-7. Under inflammatory microenvironment-mimicking conditions induced by TNFα, the extracts slightly reduced MCF-7 cell viability in a dose-dependent manner. Additionally, the extracts sensitized lapatinib-induced cytotoxicity by increasing apoptotic cell populations. Mechanistically, co-treatment with the extracts attenuated AKT and NF-kappaB activation in a dose-dependent manner.
These findings highlight the potential of P. indusiatus extracts as supplementary food for breast cancer patients, particularly in inflammatory microenvironment conditions.
评估竹荪提取物的抗炎和抗癌作用，特别是在调节炎症微环境以及使乳腺癌细胞对拉帕替尼诱导的细胞毒性敏感化方面的作用。
使用新鲜和干燥标本通过水提取法制备竹荪提取物。通过测量RAW246.7细胞中一氧化氮（NO）的产生来评估抗炎作用，同时在MCF-7和MCF-10A乳腺癌细胞系中评估细胞毒性和增殖作用。为模拟乳腺癌的炎症微环境，用肿瘤坏死因子α（TNFα）处理MCF-7细胞以诱导拉帕替尼耐药。在模拟炎症微环境的条件下评估提取物对细胞活力、凋亡和核因子κB（NF-κB）信号传导的影响。分析拉帕替尼与提取物联合处理的协同细胞毒性作用和信号通路调节。
竹荪提取物通过降低RAW246.7细胞中NO的产生表现出抗炎作用。提取物对MCF-7和MCF-10A细胞无细胞毒性，观察到MCF-10A细胞增殖增加而MCF-7细胞未增加。在TNFα诱导的模拟炎症微环境条件下，提取物以剂量依赖方式轻微降低MCF-7细胞活力。此外，提取物通过增加凋亡细胞群体使拉帕替尼诱导的细胞毒性敏感化。从机制上讲，与提取物联合处理以剂量依赖方式减弱AKT和NF-κB的激活。
这些发现突出了竹荪提取物作为乳腺癌患者补充食物的潜力,特别是在炎症微环境条件下。
