Zhang XinYu, Li Wei, Li Hongji, Xuan Xiuwei, Li Cuiping, Li Mingji
Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin Key Laboratory of Life and Health Detection, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin, 300384, PR China.
Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin Key Laboratory of Life and Health Detection, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin, 300384, PR China.
Biosens Bioelectron. 2025 Jun 15;278:117282. doi: 10.1016/j.bios.2025.117282. Epub 2025 Feb 21.
Dopamine (DA) can serve as a biomarker to evaluate emotional and cognitive disorders; however, the correlation between transient and steady-state DA levels in the brain remains unclear. In this study, gold and platinum co-doped vertical graphene (AuPt-VG) is deposited onto the surface of carbon fibers (CFs) to prepare flexible neural microwires for DA detection. The diameter of the AuPt-VG/CFs is approximately 8.5 ± 0.5 μm. When using approximately thirty microwires, a detection limit of 7.6 nM is achieved in fast-scan cyclic voltammetry (FSCV). Owing to its excellent biocompatibility, antipollution ability, high selectivity, and sensitivity, the AuPt-VG microelectrode can be implanted in rats to detect DA in the blood and brain. A correlation model between differential pulse voltammetry and FSCV is established using the AuPt-VG microelectrodes, and an electrochemical analysis software is developed to track steady-state and transient DA levels in rat and PC12 nerve cells. The electrochemical test box using the AuPt-VG microelectrode provides a new method for rapid in vitro analysis of DA levels. With the adjustable performance and high biocompatibility, AuPt-VG/CFs demonstrate excellent potential for the selective detection of neurotransmitters in the blood and brain.
多巴胺（DA）可作为评估情绪和认知障碍的生物标志物；然而，大脑中瞬态和稳态DA水平之间的相关性仍不清楚。在本研究中，将金和铂共掺杂的垂直石墨烯（AuPt-VG）沉积在碳纤维（CFs）表面，制备用于检测DA的柔性神经微丝。AuPt-VG/CFs的直径约为8.5±0.5μm。使用约三十根微丝时，在快速扫描循环伏安法（FSCV）中实现了7.6 nM的检测限。由于其优异的生物相容性、抗污染能力、高选择性和灵敏度，AuPt-VG微电极可植入大鼠体内以检测血液和大脑中的DA。使用AuPt-VG微电极建立了差分脉冲伏安法和FSCV之间的相关模型，并开发了一种电化学分析软件来跟踪大鼠和PC12神经细胞中的稳态和瞬态DA水平。使用AuPt-VG微电极的电化学测试盒为快速体外分析DA水平提供了一种新方法。凭借可调节的性能和高生物相容性，AuPt-VG/CFs在选择性检测血液和大脑中的神经递质方面显示出优异的潜力。
