Moolsup Furoida, Suttithumsatid Wiwit, Woonnoi Wanwipha, Chonpathompikunlert Pennapa, Tanasawet Supita, Sukketsiri Wanida
Laboratory Animal Service Center, Faculty of Science, Prince of Songkla University, Songkhla, Thailand.
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, Thailand.
Cell Biochem Biophys. 2025 Sep;83(3):3249-3263. doi: 10.1007/s12013-025-01706-5. Epub 2025 Mar 1.
Hepatic steatosis, commonly referred to as fatty liver disease, is defined by the abnormal buildup of fat within liver cells. Currently, primary treatments mainly focus on lifestyle changes, underscoring a lack of direct pharmacological options. Passion fruit seed extract (PFSE) has been reported to decrease hepatosteatosis; however, the mechanism underlying this effect has not been clarified. Therefore, the objective of this research was to investigate the effects and mechanisms of action of PFSE against oleic acid (OA)-induced hepatosteatosis in HepG2 cells. OA-induced HepG2 cells were analyzed by using various cell-based experiments, including assessments of cytotoxicity, reactive oxygen species (ROS) production, apoptosis, and protein and gene expression. LC-MS-MS analysis showed that PFSE contains a variety of phytochemical compounds such as alkaloids, flavonoids, stilbenoids, coumarins, terpenoids, lipids, and fatty acid derivatives, which have the potential to exhibit various pharmacological activities. In this study, PFSE demonstrated antioxidant, anti-inflammatory, and lipid metabolism-regulating activities. It also influenced key genes related to lipid metabolism, including SREBP-1c, ACC, FASN, PPARα, CPT-1A, LPL, SCD1, and LDLR. The positive effects of PFSE on OA-induced hepatic steatosis in HepG2 cells were modulated through the Akt and ERK signaling pathways, suggesting that PFSE may offer a comprehensive approach to managing hepatic steatosis.
肝脂肪变性，通常称为脂肪肝疾病，其定义为肝细胞内脂肪异常堆积。目前，主要治疗方法主要集中在生活方式的改变上，这突出表明缺乏直接的药物治疗选择。据报道，西番莲籽提取物（PFSE）可减轻肝脂肪变性；然而，这种作用的潜在机制尚未阐明。因此，本研究的目的是探讨PFSE对油酸（OA）诱导的HepG2细胞肝脂肪变性的影响及其作用机制。通过各种基于细胞的实验对OA诱导的HepG2细胞进行分析，包括细胞毒性评估、活性氧（ROS）生成、细胞凋亡以及蛋白质和基因表达。液相色谱-串联质谱（LC-MS-MS）分析表明，PFSE含有多种植物化学化合物，如生物碱、黄酮类化合物、芪类化合物、香豆素、萜类化合物、脂质和脂肪酸衍生物，它们具有展现各种药理活性的潜力。在本研究中，PFSE表现出抗氧化、抗炎和脂质代谢调节活性。它还影响与脂质代谢相关的关键基因，包括固醇调节元件结合蛋白-1c（SREBP-1c）、乙酰辅酶A羧化酶（ACC）、脂肪酸合酶（FASN）、过氧化物酶体增殖物激活受体α（PPARα）、肉碱棕榈酰转移酶-1A（CPT-1A）、脂蛋白脂肪酶（LPL）、硬脂酰辅酶A去饱和酶1（SCD1）和低密度脂蛋白受体（LDLR）。PFSE对OA诱导的HepG2细胞肝脂肪变性的积极作用是通过Akt和ERK信号通路调节的，这表明PFSE可能为管理肝脂肪变性提供一种综合方法。
