通过过表达Pdx1从大鼠脂肪组织间充质干细胞生成的胰岛素生成细胞会激活免疫反应。 （原英文句子表述似乎不完整，“both and”后面缺少内容，但仅按现有内容翻译如上）
The Insulin-Producing Cells Generated from Rat Adipose Tissue Mesenchymal Stem Cells via Pdx1 Overexpression Activate an Immune Response both and .
作者信息
Mohammadian Shadab, Hosseni Seyed Javad, Negad Dehbashi Fereshte, Dayer Dian
机构信息
Group of Biotechnology, Institute of Persian Gulf, Persian Gulf University, Bushehr, Iran.
Department of Biological Science and Technology, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, Iran.
出版信息
Iran J Med Sci. 2025 Feb 1;50(2):112-123. doi: 10.30476/ijms.2024.101162.3378. eCollection 2025 Feb.
BACKGROUND
The current work investigated the immunological features of insulin-producing cells (IPCs) generated from rat adipose-derived mesenchymal stem cells (ADSCs) both and .
METHODS
The research was carried out at Ahvaz Jundishapur University of Medical Sciences in 2023. ADSCs were derived from rat adipose tissues and differentiated into IPCs. The control group included undifferentiated ADSCs. The amount of secreted insulin was measured using ELISA. The expression of major histocompatibility complex-I (MHC-I) and MHC-II, cluster of differentiation 40 (CD40), and CD80 by IPCs was assessed using Western Blot analysis. The study was performed on 10 male diabetic rats. The experimental group received 10 IPCs in the peritoneal cavity. The control group received 10 undifferentiated ADSCs. After 4 hours, the expression of CD3a and CD45 by immune cells collected from the peritoneal cavity was measured using flow cytometry. All parameters were statistically analyzed using a test.
RESULTS
The differentiated cells secreted much higher amounts of insulin than the control group (P=0.04). IPCs exhibited higher expression of MHC-I and MHC-II, CD40, and CD80 (P=0.02, P=0.008, P=0.07, and P=0.02, respectively). The experimental group showed higher levels of CD3a and CD45 expression than the control group (P=0.07, P=0.04, respectively).
CONCLUSION
Functional IPCs generated by ADSCs differentiation exhibited immunogenic activity both and . Immune-modulating strategies are required for the effective transplantation of the differentiated IPCs generated in our study.
背景
当前研究调查了大鼠脂肪来源间充质干细胞（ADSCs）生成的胰岛素产生细胞（IPCs）的免疫特性。
方法
该研究于2023年在阿瓦士·贾undishapur医科大学开展。ADSCs取自大鼠脂肪组织并分化为IPCs。对照组包括未分化的ADSCs。使用酶联免疫吸附测定法（ELISA）测量分泌的胰岛素量。通过蛋白质免疫印迹分析评估IPCs的主要组织相容性复合体-I（MHC-I）和MHC-II、分化簇40（CD40）以及CD80的表达。该研究在10只雄性糖尿病大鼠身上进行。实验组在腹腔内注射10个IPCs。对照组注射10个未分化的ADSCs。4小时后，使用流式细胞术测量从腹腔收集的免疫细胞中CD3a和CD45的表达。所有参数均使用t检验进行统计学分析。
结果
分化细胞分泌的胰岛素量比对照组高得多（P = 0.04）。IPCs表现出更高的MHC-I和MHC-II、CD40以及CD80表达（分别为P = 0.02、P = 0.008、P = 0.07和P = 0.02）。实验组的CD3a和CD45表达水平高于对照组（分别为P = 0.07、P = 0.04）。
结论
ADSCs分化产生的功能性IPCs在体内和体外均表现出免疫原性活性。对于我们研究中产生的分化IPCs的有效移植，需要免疫调节策略。
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