Karácsonyi S, Mándi Y, Farkas G, Tóth M, Béládi I
Acta Chir Hung. 1985;26(1):51-8.
The NK (natural killer) and K (killer) activities of peripheral lymphocytes were determined. The peripheral blood was obtained from healthy individuals and from patients with liver and pancreas diseases. Examinations were performed preoperatively. The natural cell-dependent cytotoxicity (NK) was examined against the K-562 cell line, while the antibody-dependent cellular cytotoxicity (ADCC) was examined against human red blood cells. The NK and K cell activities of the 19 patients with malignant tumours were substantially lower than those of the 40 healthy subjects. In the 12 cases of benign diseases of the liver and pancreas, however, the mean percentage cytotoxicity expressing the NK and K activities (40 +/- 10%) agreed with, or was higher than, the value for the controls (32 +/- 12%). Low cytotoxicity levels (12.6 +/- 5%) were characteristic in the malignant processes; in the majority of these, exploration showed local metastases or metastases besides the primary lesion. Since the NK and K cells play an essential role in the defence of the organism against tumours, the decrease in activity of this cell population may be of diagnostic value. In vitro interferon treatment in the control and benign cases considerably stimulated the NK reaction, and in some of the malignant diseases it raised the cytotoxicity values close to the control level.
检测了外周淋巴细胞的NK（自然杀伤）和K（杀伤）活性。外周血取自健康个体以及患有肝脏和胰腺疾病的患者。术前进行检查。针对K-562细胞系检测自然细胞依赖性细胞毒性（NK），而针对人红细胞检测抗体依赖性细胞毒性（ADCC）。19例恶性肿瘤患者的NK和K细胞活性显著低于40名健康受试者。然而，在12例肝脏和胰腺良性疾病患者中，表达NK和K活性的平均细胞毒性百分比（40±10%）与对照组（32±12%）的值相符或更高。低细胞毒性水平（12.6±5%）是恶性病变的特征；在大多数此类病例中，探查显示除原发灶外还存在局部转移或远处转移。由于NK和K细胞在机体抵御肿瘤的防御中起重要作用，该细胞群体活性的降低可能具有诊断价值。在对照组和良性病例中，体外干扰素治疗显著刺激了NK反应，在一些恶性疾病中，它使细胞毒性值提高至接近对照水平。
