Lui A, Lumeng L, Li T K
Am J Clin Nutr. 1985 Jun;41(6):1236-43. doi: 10.1093/ajcn/41.6.1236.
A cation-exchange high-performance liquid chromatographic (HPLC) method was found to be comparable to the open-column (OCC) method for measuring six different B6 compounds in human plasma and the L-tyrosine apodecarboxylase (LTD) assay for pyridoxal-P (PLP). Plasma samples were obtained from 9 subjects before and after 7 days of pyridoxine (PN) supplementation. PLP, pyridoxal (PL) and 4-pyridoxic acid (4-PA) were the major B6 compounds in plasma and the only compounds which increased after supplementation. The coefficients of correlation between any 2 of the 3 methods in measuring plasma PLP were greater than 0.93, and between HPLC and OCC in quantifying PL and 4-PA were 0.82 and 0.63, respectively. With the low plasma levels of pyridoxamine-P, PN and pyridoxamine, the results from OCC were consistently higher than those from HPLC. However, recoveries of spiked B6 compounds in plasma by these methods were between 84 to 105 percent for all the 5 vitamers and 4-PA.
已发现一种阳离子交换高效液相色谱（HPLC）法在测量人体血浆中六种不同维生素B6化合物以及用于测定磷酸吡哆醛（PLP）的L-酪氨酸脱羧酶（LTD）分析方面，与开柱（OCC）法相当。从9名受试者在补充吡哆醇（PN）7天前后采集血浆样本。PLP、吡哆醛（PL）和4-吡哆酸（4-PA）是血浆中的主要维生素B6化合物，也是补充后仅有的增加的化合物。三种方法中任意两种在测量血浆PLP时的相关系数均大于0.93，HPLC和OCC在定量PL和4-PA时的相关系数分别为0.82和0.63。由于血浆中磷酸吡哆胺、PN和吡哆胺的水平较低，OCC的结果始终高于HPLC的结果。然而，这些方法对血浆中添加的维生素B6化合物的回收率，对于所有5种维生素和4-PA而言，在84%至105%之间。
