Alves-Coêlho Tássio, Chagas-de-Souza Darlison, Fernandes Chagas Carolina Romeiro, Gutiérrez-Liberato Germán Alfredo, Perles Lívia, Alabi Amir, André Marcos Rogério, Viana Lúcio André
Laboratory of Morphophysiological and Parasitic Studies, Department of Biological and Health Sciences, Federal University of Amapá, 68903-419, Macapá, Amapá, Brazil.
Postgraduate Program in Tropical Biodiversity, Federal University of Amapá, 68903-419, Macapá, Amapá, Brazil.
Int J Parasitol Parasites Wildl. 2025 Feb 4;26:101044. doi: 10.1016/j.ijppaw.2025.101044. eCollection 2025 Apr.
Here we describe, in an unprecedented way for the Brazilian Amazon, one Apicomplexa species infecting an anuran in Pará, Brazil, using an integrative approach that includes taxonomy, providing morphology, morphometrics, and molecular data. Samples were collected between February 2021 and February 2022 in the community of Curupira, municipality of Santarém, and in the industrial area of Marabá, both in Pará state. Specimens of and were captured by hand during the active search, and blood samples were collected by cardiac puncture. One blood aliquot was used to prepare blood smears for microscopical analysis and another aliquot for further molecular analyses. Of the five caught, one (20%) presented parasites morphologically compatible with the parasites belonging to the genus , while of the three caught, one (33.3%) presented parasites morphologically compatible with the parasites belong to the genera and . Based on molecular data of rDNA sequences, the sequence obtained clustered with other parasite species recovered from Brazilian amphibians. The sp. sequence was placed in a sister clade of species described in birds and close to , described in amphibians. All attempts to amplify parasite DNA were unsuccessful. We described n. sp. and reported infection by sp. and sp. in bufonids anurans from the Brazilian Amazon. This study increases knowledge of Brazilian anuran hemoparasites and confirms the importance of using an integrative approach for the taxonomy of these parasite groups.
在这里，我们以前所未有的方式描述了巴西亚马逊地区一种感染巴西帕拉州一只无尾两栖动物的顶复门物种，采用了一种综合方法，包括分类学，提供形态学、形态测量学和分子数据。样本于2021年2月至2022年2月在圣塔伦市库鲁皮拉社区和马拉巴工业区采集，这两个地方都位于帕拉州。在主动搜索过程中手工捕获了蟾蜍和雨蛙标本，并通过心脏穿刺采集血样。一份血样用于制备血涂片进行显微镜分析，另一份用于进一步的分子分析。在捕获的5只蟾蜍中，有1只（20%）呈现出形态上与属于嗜组织抱子虫属的寄生虫相符的寄生虫，而在捕获的3只雨蛙中，有1只（33.3%）呈现出形态上与属于嗜组织抱子虫属和疟原虫属的寄生虫相符的寄生虫。基于嗜组织抱子虫核糖体DNA序列的分子数据，获得的嗜组织抱子虫序列与从巴西两栖动物中分离出的其他寄生虫物种聚类。嗜组织抱子虫属物种序列位于从鸟类中描述的嗜组织抱子虫属物种的姐妹分支中，并与从两栖动物中描述的疟原虫属接近。所有扩增疟原虫属寄生虫DNA的尝试均未成功。我们描述了嗜组织抱子虫新种，并报告了巴西亚马逊地区蟾蜍科无尾两栖动物感染嗜组织抱子虫属物种和疟原虫属物种的情况。这项研究增加了对巴西无尾两栖动物血液寄生虫的了解，并证实了使用综合方法对这些寄生虫类群进行分类的重要性。
