Bilhalva Lina Crespo, de Almeida Bruno Albuquerque, Colombo Patrick, de Faria Valle Stella, Soares João Fabio
Department of Veterinary Clinical Pathology, Veterinary Medicine College, Federal University of Rio Grande do Sul (UFRGS), 9090 Bento Gonçalves Ave., Porto Alegre 91540-000, Rio Grande do Sul, Brazil.
Zoobotanical Foundation of Rio Grande do Sul (FZB-RS), 1427 Dr. Salvador França Ave., Porto Alegre 90690-000, Rio Grande do Sul, Brazil.
Vet Parasitol Reg Stud Reports. 2023 Feb;38:100834. doi: 10.1016/j.vprsr.2023.100834. Epub 2023 Jan 7.
It has been suggested that anuran amphibian parasites can cause clinical signs in situations of environmental imbalance. In the family Leptodactylidae, information about hematology is scarce, although these are well-known tools for the diagnosis and prognosis in clinical practice and potential bioindicators of environmental stress. The objective of this study is to describe Leptodactylus luctator hematology, to report the occurrence of hemoparasites and thrombidiform mites, and to compare the hematological variables under the presence and absence of these organisms. Ectoparasites and heparinized blood samples from 40 free-living specimens of L. luctator were collected for analysis. Hematologic variables and total plasma protein were compared between groups with and without hemoparasites and intradermal mites. As results, structures compatible with hemogregarines, Lankesterella sp., five morphotypes of Trypanosoma spp., microfilaria, Aegyptianella sp., an unidentified intraleukocytic hemoparasite, and frog erythrocytic virus (FEV) inclusion bodies were identified in the blood samples, besides Hannemania spp. intradermal mites. The hemoparasite occurrence was higher than previously reported in other anuran families and locations. Also, L. luctator has smaller red blood cells (RBCs) and white blood cells (WBCs), and a hyposegmentation of the neutrophil nucleus, when compared to many other amphibians. White blood cell, neutrophil, and monocyte counts were higher in animals parasitized by mites. There was no correlation between the number of parasitized RBCs and hematologic variables. This study provides anuran hematologic information, in addition to indicating a host reaction to infestation by Hannemania spp. mites, besides constituting the first record of the distribution of hemoparasites and intradermal mites in L. luctator of the study region.
有人认为，在环境失衡的情况下，无尾两栖动物寄生虫会引发临床症状。在细趾蟾科中，血液学方面的信息匮乏，尽管这些信息在临床实践中是用于诊断和预后的重要工具以及环境压力的潜在生物指标。本研究的目的是描述黑眼先蟾的血液学特征，报告血寄生虫和血栓样螨的出现情况，并比较有或没有这些生物体存在时的血液学变量。采集了40只自由生活的黑眼先蟾的体表寄生虫和肝素化血液样本进行分析。比较了有或没有血寄生虫和皮内螨的组之间的血液学变量和总血浆蛋白。结果显示，在血液样本中除了汉氏螨属皮内螨外，还鉴定出了与血簇虫、兰氏血簇虫属、五种锥虫属形态型、微丝蚴、埃及小体属、一种未鉴定的白细胞内血寄生虫以及蛙红细胞病毒（FEV）包涵体相符的结构。血寄生虫的出现率高于此前在其他无尾两栖动物科和地点所报告的。此外 , 与许多其他两栖动物相比，黑眼先蟾的红细胞和白细胞较小，中性粒细胞核分叶过少。被螨寄生的动物白细胞、中性粒细胞和单核细胞计数较高。被寄生的红细胞数量与血液学变量之间没有相关性。本研究提供了无尾两栖动物的血液学信息，除了表明宿主对汉氏螨属螨感染的反应外，还构成了研究区域黑眼先蟾血寄生虫和皮内螨分布的首次记录。
