Khoswanto Christian, Dewi Ira Kusuma
Department of Oral Biology Faculty of Dentistry, Airlangga University, Surabaya, Indonesia.
Dentistry Clinic Research, Surabaya, Indonesia.
J Oral Biol Craniofac Res. 2025 Mar-Apr;15(2):359-364. doi: 10.1016/j.jobcr.2025.02.001. Epub 2025 Feb 13.
Dentists frequently conduct tooth extractions when there is damage to the tooth or the tissue that supports it. When a tooth is extracted, the alveolar bone will sustain injury. Most of the initial bone volume is lost following the healing phase after extraction. Rehabilitation must start as soon as a tooth is missing, mainly because the alveolar bone is severely damaged during the tooth extraction, particularly in the buccal region where the tooth is removed. Dental implant is one method of replacing lost teeth. One of the most important elements influencing the clinical result of dental implants is a change in the dimension of the alveolar bone. Several bone-grafting techniques, such as socket preservation techniques, have been developed to increase the volume of bone throughout the healing phase after tooth extraction. This study aims to assess the impact rhBMP-2 on creating bone regeneration through VEGF and HIF-1α in the mandibular socket post-extraction in Wistar rats.
On the anterior side of the mandible, in the socket area where the tooth was extracted, rhBMP-2 was injected into the socket, and the xenograft material was applied with a syringe. Male, 9-week-old Wistar rats were chosen (n = 30).
Our statistical evaluations have revealed a significantly higher VEGF-A and HIF-1α expression post-extraction of the rhBMP-2 and xenograft group compared to other group treatments. These findings are significant as they provide a deeper understanding of the mechanisms involved in bone regeneration post-extraction.
Our study suggests that injecting rhBMP-2 into the grafted material and socket extraction during GBR dramatically increases the expression of VEGF-A and HIF-1α. These findings have the potential to significantly impact oral surgery and regenerative dentistry, opening up new possibilities for enhancing bone regeneration techniques.
当牙齿或其支持组织受损时，牙医经常进行拔牙操作。拔牙时，牙槽骨会受到损伤。拔牙后的愈合阶段，大部分初始骨量会流失。牙齿缺失后必须尽快开始修复，主要是因为拔牙过程中牙槽骨会受到严重损伤，尤其是在拔牙的颊侧区域。牙种植是一种修复缺失牙的方法。影响牙种植临床效果的最重要因素之一是牙槽骨尺寸的变化。已经开发了几种骨移植技术，如牙槽窝保存技术，以在拔牙后的整个愈合阶段增加骨量。本研究旨在评估重组人骨形态发生蛋白-2（rhBMP-2）通过血管内皮生长因子（VEGF）和缺氧诱导因子-1α（HIF-1α）对Wistar大鼠下颌牙槽窝拔牙后骨再生的影响。
在Wistar大鼠下颌骨前侧拔牙的牙槽窝区域，将rhBMP-2注入牙槽窝，并使用注射器施加异种移植材料。选用9周龄雄性Wistar大鼠（n = 30）。
我们的统计评估显示，与其他组治疗相比，rhBMP-2和异种移植组拔牙后VEGF-A和HIF-1α表达显著更高。这些发现具有重要意义，因为它们为拔牙后骨再生所涉及的机制提供了更深入的理解。
我们的研究表明，在引导骨再生（GBR）过程中，将rhBMP-2注入移植材料和牙槽窝拔牙部位可显著增加VEGF-A和HIF-1α的表达。这些发现有可能对口腔外科和再生牙科产生重大影响，为增强骨再生技术开辟新的可能性。
