Forker E L, Luxon B A
Am J Physiol. 1985 Jun;248(6 Pt 1):G709-17. doi: 10.1152/ajpgi.1985.248.6.G709.
Proceeding from the observation that organic anions bound to albumin have hepatic extraction fractions that are unexpectedly high, we have studied a distributed model that accounts for this phenomenon by invoking sites on the cell surface that catalyze the dissociation of albumin-anion complexes. The present report extends this model to include nonequilibrium binding and rate-limiting diffusion of bound anion to the cell surface. Simulation analysis of the extended model provides an unambiguous basis for interpreting the apparent intrinsic clearance of free anion. The model is fully consistent with the transport data that we obtained with rose bengal [Am. J. Physiol. 248 (Gastrointest. Liver Physiol. 11): G702-G708, 1985] but is suited to estimating only selected components of the unknown parameter vector. Uncertainties inherent in an alternate approach are discussed and illustrated.
基于有机阴离子与白蛋白结合后肝脏摄取分数出奇地高这一观察结果，我们研究了一种分布式模型，该模型通过调用细胞表面催化白蛋白 - 阴离子复合物解离的位点来解释这一现象。本报告将该模型扩展到包括非平衡结合以及结合阴离子向细胞表面的限速扩散。扩展模型的模拟分析为解释游离阴离子的表观内在清除率提供了明确的依据。该模型与我们用孟加拉玫瑰红获得的转运数据[《美国生理学杂志》248卷（胃肠肝脏生理学11）：G702 - G708，1985]完全一致，但仅适用于估计未知参数向量的选定成分。讨论并举例说明了另一种方法中固有的不确定性。
