Forker E L, Luxon B A, Sharma V S
Am J Physiol. 1985 Jun;248(6 Pt 1):G702-8. doi: 10.1152/ajpgi.1985.248.6.G702.
Gamma globulin and albumin are compared with respect to their effects on the hepatic transport of rose bengal and with respect to the rates and affinities with which they bind this dye. The apparent intrinsic clearance of rose bengal is greater in the presence of albumin than in the presence of gamma globulin, and this difference increases with the protein concentration. Because the binding affinities of these proteins also differ, however, it cannot be concluded decisively that the mechanisms of dye removal are distinct. For both proteins the binding reaction rates as measured by stopped-flow spectrophotometry are much faster than the rate of convection along the sinusoid or the rate of removal of free dye by liver cells. The transport data and the binding rate constants are the basis for an extended theoretical model developed and analyzed in an accompanying report.
比较了γ球蛋白和白蛋白对孟加拉玫瑰红肝脏转运的影响，以及它们结合这种染料的速率和亲和力。在白蛋白存在下，孟加拉玫瑰红的表观内在清除率高于γ球蛋白存在下的情况，且这种差异随蛋白质浓度增加而增大。然而，由于这些蛋白质的结合亲和力也不同，所以不能确凿地得出染料清除机制不同的结论。对于这两种蛋白质，通过停流分光光度法测得的结合反应速率比沿肝血窦的对流速率或肝细胞清除游离染料的速率快得多。转运数据和结合速率常数是随附报告中开发和分析的扩展理论模型的基础。
