de Léo Rosa Aparecida Reis, Valentim Jean Kaique, de Sousa Ferreira Rafael, de França Thaisa Pereira, Ribeiro Artur Macedo, Gomes Kaique Moreira, de Almeida Alexander Alexandre, Calderano Arele Arlindo
Department of Animal Sciences, Federal University of Viçosa (UFV), Avenue: P H Rolfs, S/N - Campus Universitário, Viçosa, Minas Gerais, Brazil.
Faculty of Agricultural Sciences, Federal University of Grande Dourados (UFGD), Highway Dourados - Itahum, Km 12. Cidade Universitária, Dourados, Mato Grosso Do Sul, Brazil.
Trop Anim Health Prod. 2025 Mar 4;57(2):93. doi: 10.1007/s11250-025-04343-5.
The presence of phytate in broiler diets restricts phosphorus availability, a crucial nutrient for muscle and bone development, leading to reduced performance. To alleviate this issue, exogenous enzymes such as phytase are introduced; however, the wide variety of phytases available on the market and their effectiveness are not yet fully understood. The objective of this study was to evaluate the phosphorus equivalence of commercial phytase in broiler diets, using the standard curve, and its effects on performance, bone mineralization, intestinal histological integrity, liver relative weight, and total alkaline phosphatase in the initial phase (1 to 21 days) of broiler. We used 2,500 male broiler chickens, with an initial weight of 44 g, distributed in a completely randomized design with 10 treatments and 10 replications of 25 birds per experimental unit. The diets were formulated to meet the nutritional requirements of the birds, except for the available phosphorus (Pd) and calcium content. The treatments were divided as follows: T1-Control (CT) with 0.18% Pd; T2-CT + 0.07% aP; T3-CT + 0.14% aP; T4-CT + 0.21% aP; T5-CT + phytase 1; T6-CT + phytase 2; T7-CT + phytase 3; T8-CT + phytase 4; T9-CT + phytase 5; and T10-CT + phytase 6. For enzymatic standardization, 500 FTU/kg (Phytase Units) of each enzyme were used. Animals subjected to the highest levels of P (T2, T3, and T4) and with the addition of commercial phytases (T5, T6, T7, T8, T9, and T10) had better performance and bone mineralization results when compared to T1-CT. All phytases evaluated improved final live weight, weight gain, feed conversion, and ash and phosphorus content in the tibia and intestinal development compared to CT. Supplementing broiler diets with commercial phytases enhances performance, bone mineralization, and jejunal development from 1 to 21 days.The average phosphorus availability from phytic acid in these phytases was 0.154%. The comparison of the six commercial phytases in diets for broiler chickens, using the standard curve and supplementation with 25 g/t of commercial phytase, showed similar results, promoting improvements in performance, bone mineralization, and jejunal development.
肉鸡日粮中植酸的存在会限制磷的有效性，而磷是肌肉和骨骼发育的关键营养素，会导致生产性能下降。为缓解这一问题，引入了植酸酶等外源酶；然而，市场上植酸酶种类繁多，其有效性尚未完全明确。本研究的目的是使用标准曲线评估商业植酸酶在肉鸡日粮中的磷当量，及其对肉鸡初始阶段（1至21天）生产性能、骨矿化、肠道组织学完整性、肝脏相对重量和总碱性磷酸酶的影响。我们使用了2500只初始体重为44克的雄性肉鸡，采用完全随机设计，分为10个处理组，每个实验单元有10个重复，每个重复25只鸡。日粮的配方旨在满足鸡的营养需求，但可利用磷（Pd）和钙含量除外。处理组划分如下：T1-对照组（CT），含0.18% Pd；T2-CT + 0.07% 无机磷（aP）；T3-CT + 0.14% aP；T4-CT + 0.21% aP；T5-CT + 植酸酶1；T6-CT + 植酸酶2；T7-CT + 植酸酶3；T8-CT + 植酸酶4；T9-CT + 植酸酶5；T10-CT + 植酸酶6。为进行酶标准化，每种酶使用500 FTU/kg（植酸酶单位）。与T1-CT相比，接受最高磷水平（T2、T3和T4）以及添加商业植酸酶（T5、T6、T7、T8、T9和T10）的动物具有更好的生产性能和骨矿化结果。与对照组相比，所有评估的植酸酶均提高了最终活重、体重增加、饲料转化率以及胫骨中的灰分和磷含量，促进了肠道发育。在1至21天内，给肉鸡日粮补充商业植酸酶可提高生产性能、骨矿化和空肠发育。这些植酸酶中植酸的平均磷利用率为0.154%。使用标准曲线并添加25克/吨商业植酸酶，对肉鸡日粮中的六种商业植酸酶进行比较，结果相似，均促进了生产性能、骨矿化和空肠发育的改善。
