6-羟基苯并噻唑对辣根过氧化物酶催化的环状二酰肼化学发光氧化反应的增强作用。
Enhancement of the horseradish peroxidase-catalyzed chemiluminescent oxidation of cyclic diacyl hydrazides by 6-hydroxybenzothiazoles.
作者信息
Thorpe G H, Kricka L J, Gillespie E, Moseley S, Amess R, Baggett N, Whitehead T P
出版信息
Anal Biochem. 1985 Feb 15;145(1):96-100. doi: 10.1016/0003-2697(85)90332-x.
6-Hydroxybenzothiazole, 2-cyano-6-hydroxybenzothiazole, and 2-(6-hydroxy-2-benzothiazolyl)thiazole-4-carboxylic acid (dehydroluciferin) dramatically enhance light emission from the horseradish peroxidase conjugate catalyzed oxidation of luminol, isoluminol, N-(6-aminobutyl)-N-ethyl isoluminol, and 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide by either peroxide or perborate. Light emission is enhanced by up to 1000-fold, which is an improvement over the enhancement previously observed using firefly luciferin (4,5-dihydro-2-(6-hydroxy-2-benzothiazolyl)thiazole-4-carboxylic acid). Enhancement is influenced by enhancer concentration and pH. Spectral scans of light emitted in enhanced and unenhanced reactions are similar, suggesting that aminophthalate products, and not the enhancers, are the emitters.
6-羟基苯并噻唑、2-氰基-6-羟基苯并噻唑和2-(6-羟基-2-苯并噻唑基)噻唑-4-羧酸（脱氢荧光素）可显著增强辣根过氧化物酶共轭物催化鲁米诺、异鲁米诺、N-(6-氨基丁基)-N-乙基异鲁米诺以及7-二甲基氨基萘-1,2-二羧酸酰肼被过氧化物或过硼酸盐氧化时的发光。发光增强可达1000倍，相较于之前使用萤火虫荧光素（4,5-二氢-2-(6-羟基-2-苯并噻唑基)噻唑-4-羧酸）所观察到的增强效果有所改善。增强效果受增强剂浓度和pH值影响。增强反应和未增强反应中发射光的光谱扫描相似，这表明氨基邻苯二甲酸产物而非增强剂是发光体。
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