Thorpe G H, Kricka L J
Methods Enzymol. 1986;133:331-53. doi: 10.1016/0076-6879(86)33078-7.
Enhancement of light emission from the horseradish peroxidase-catalyzed oxidation of diacyl hydrazides on addition of 6-hydroxybenzothiazole or phenol derivatives forms the basis of rapid, specific, and sensitive chemiluminescent assays for peroxidase. The advantages and wide applicability of the technique have been demonstrated in a range of ligand-binding assays. Careful selection of chemiluminescent reagents, enhancer, their relative proportions and reaction conditions, and more detailed knowledge of the mechanism of enhancement should enable further improvements in sensitivity and the intensity or constancy of light emission.
在添加6-羟基苯并噻唑或酚类衍生物的情况下，辣根过氧化物酶催化二酰肼氧化时发光增强，这构成了过氧化物酶快速、特异且灵敏的化学发光测定法的基础。该技术的优势和广泛适用性已在一系列配体结合测定中得到证实。仔细选择化学发光试剂、增强剂、它们的相对比例和反应条件，以及对增强机制更详细的了解，应能进一步提高灵敏度以及发光强度或稳定性。
