Reyes Guillermo, Betancourt Irma, Andrade Betsy, Pozo Yessenia, Sorroza Lita, Trujillo Luis E, Bayot Bonny
Centro Nacional de Acuicultura E Investigaciones Marinas, CENAIM, Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil, Ecuador.
Facultad de Ciencias Agropecuarias, Universidad Técnica de Machala, 5.5 Av Panamericana, Machala, Ecuador.
BMC Res Notes. 2025 Mar 4;18(1):97. doi: 10.1186/s13104-025-07162-x.
This study highlighted the gap in the genetic characterization of marine bacteria, specifically within the genus Thiohalocapsa. This genus thrives in contaminated environments with high concentrations of sulfide, such as treated municipal wastewater. Thc. marina is a phototrophic purple bacterium known for its role in sulfur oxidation and bioremediation in marine aquaculture systems. To date, only one Thc. marina genome has been published in the GenBank database. This study enhances the understanding of the ecological adaptation and bioremediation capabilities of Thc. marina in treated municipal wastewater effluents.
We present a draft genome of Thc. marina LNA26 recovered from treated municipal wastewater effluents using shotgun metagenomic sequencing. The genome of Thc. marina LNA26 harbors 4,356,720 bp and contains 4,032 genes (3,936 CDSs, 50 RNA genes, and 46 pseudogenes), some of them involved in sporulation, siderophores biosynthesis, arsenate bioremediation, sulfide metabolism, capacity for nitrogen fixation, the biosynthesis of PHA, and NHPL bacteriocins. Thc. marina LNA26 exhibits 3 CRISPR Arrays and a high abundance of COGs in signal transduction, energy production, and cell wall biogenesis, indicating advanced environmental responsiveness, energy efficiency, and cellular robustness.
本研究突出了海洋细菌遗传特征方面的差距，特别是在硫卤球菌属内。该属在含有高浓度硫化物的污染环境中繁衍生息，如经处理的城市污水。滨海硫卤球菌是一种光合紫色细菌，因其在海水养殖系统中的硫氧化和生物修复作用而闻名。迄今为止，GenBank数据库中仅公布了一个滨海硫卤球菌的基因组。本研究增进了对滨海硫卤球菌在经处理的城市污水中的生态适应性和生物修复能力的理解。
我们展示了通过鸟枪法宏基因组测序从经处理的城市污水中获得的滨海硫卤球菌LNA26的基因组草图。滨海硫卤球菌LNA26的基因组包含4356720个碱基对，含有4032个基因（3936个编码序列、50个RNA基因和46个假基因），其中一些基因参与孢子形成、铁载体生物合成、砷酸盐生物修复、硫化物代谢、固氮能力、聚羟基脂肪酸酯的生物合成以及NHPL细菌素的合成。滨海硫卤球菌LNA26表现出3个CRISPR阵列，并且在信号转导、能量产生和细胞壁生物合成方面有大量的直系同源基因簇，表明其具有先进的环境响应能力、能量效率和细胞稳健性。
