Anil Kumar P, Srinivas T N R, Thiel V, Tank M, Sasikala Ch, Ramana Ch V, Imhoff J F
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500 085, India.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2333-8. doi: 10.1099/ijs.0.003053-0. Epub 2009 Jul 20.
A spherical-shaped, phototrophic, purple sulfur bacterium was isolated in pure culture from anoxic sediment in a marine aquaculture pond near Bheemli (India). Strain JA142T is Gram-negative and non-motile. It has a requirement for NaCl (optimum of 2% and maximum of 6% w/v NaCl). Intracellular photosynthetic membranes are of the vesicular type. In vivo absorption spectra indicate the presence of bacteriochlorophyll a and carotenoids of the okenone series as photosynthetic pigments. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA142T is related to halophilic purple sulfur bacteria of the genera Thiohalocapsa and Halochromatium, with the highest sequence similarity to Thiohalocapsa halophila DSM 6210T (97.5%). Morphological and physiological characteristics differentiate strain JA142T from other species of the genera Halochromatium and Thiohalocapsa. Strain JA142T is sufficiently different from Thiohalocapsa halophila based on 16S rRNA gene sequence analysis and morphological and physiological characteristics to allow the proposal of a novel species, Thiohalocapsa marina sp. nov., with the type strain JA142T (=JCM 14780T=DSM 19078T).
从印度比姆利附近海水养殖池塘的缺氧沉积物中分离出一种球形光合紫色硫细菌，并进行了纯培养。菌株JA142T为革兰氏阴性，不运动。它需要NaCl（最适浓度为2%，最高浓度为6% w/v NaCl）。细胞内光合膜为泡囊型。体内吸收光谱表明存在细菌叶绿素a和奥克酮系列类胡萝卜素作为光合色素。基于16S rRNA基因序列的系统发育分析表明，菌株JA142T与硫卤球菌属和嗜盐色菌属的嗜盐紫色硫细菌相关，与嗜盐硫卤球菌DSM 6210T的序列相似性最高（97.5%）。形态学和生理学特征将菌株JA142T与嗜盐色菌属和硫卤球菌属的其他物种区分开来。基于16S rRNA基因序列分析以及形态学和生理学特征，菌株JA142T与嗜盐硫卤球菌有足够差异，因此提议将其作为一个新物种，即海洋硫卤球菌（Thiohalocapsa marina sp. nov.），模式菌株为JA142T（=JCM 14780T=DSM 19078T）。
