Bouloux P, Perrett D, Besser G M
Ann Clin Biochem. 1985 Mar;22 ( Pt 2):194-203. doi: 10.1177/000456328502200217.
We report on a systematic investigation into some frequently encountered problems in the estimation of catecholamines in plasma and biological fluids using high-performance liquid chromatography with electrochemical detection. The kinetics of adsorption and desorption of catecholamines from plasma on to alumina has been studied using laboratory-prepared and some commercial aluminas with various acids. Chromatographic conditions yielding optimal resolution and sensitivity have been characterised. Some possible interfering peaks have been identified. The stability of catecholamines in plasma has been studied.
我们报告了一项关于使用高效液相色谱-电化学检测法测定血浆和生物体液中儿茶酚胺时一些常见问题的系统研究。使用实验室制备的以及一些含不同酸的市售氧化铝，研究了儿茶酚胺从血浆吸附到氧化铝以及从氧化铝解吸的动力学。已对产生最佳分离度和灵敏度的色谱条件进行了表征。已识别出一些可能的干扰峰。还研究了儿茶酚胺在血浆中的稳定性。
