N'Diaye A, Georges P, N'Go A, Festy B
Appl Environ Microbiol. 1985 May;49(5):1072-5. doi: 10.1128/aem.49.5.1072-1075.1985.
The legal biological survey of swimming pool waters is based on both the level of bacteriological contamination and the amount of material of fecal origin. The great number of soil amoebas and the occasional epidemiological risk involved led us to consider using these organisms as possible biological markers to estimate the quality of pool water and the extent of disinfection. During a 1-year survey of 54 public swimming pools, 765 superficial pool and tap water samples were collected. One portion (50 ml) drawn from 1-liter samples was filtered and cultured for amoebas. In specimens considered contaminated we detected at least 20 amoebas per liter, whereas uncontaminated samples contained fewer than 20 amoebas per liter. By keeping the threshold value voluntarily low, we were able to compare tap water with pool water and to monitor the quality of various disinfection procedures (i.e., chlorine, bromine, and Cu-Ag). The data suggest that the filters were not always protective against a high concentration of amoebas. Furthermore, these disinfection procedures were not equally efficient according to estimates based on biological criteria. In addition, the quality of swimming pool water also depends on the quality of its source tap water. Thus, the numeration of soil amoebas can be used as an additional biological marker to estimate the quality of swimming pool water.
游泳池水的法定生物学检测基于细菌污染水平和粪便来源物质的数量。大量的土壤变形虫以及偶尔涉及的流行病学风险促使我们考虑将这些生物体用作可能的生物学标志物，以评估泳池水的质量和消毒程度。在对54个公共游泳池进行的为期1年的调查中，采集了765份泳池表面水和自来水样本。从1升样本中抽取的一部分（50毫升）经过过滤并培养变形虫。在被认为受污染的样本中，每升至少检测到20个变形虫，而未受污染的样本每升含有的变形虫少于20个。通过有意将阈值设得较低，我们能够将自来水与泳池水进行比较，并监测各种消毒程序（即氯、溴和铜银离子）的质量。数据表明，过滤器并非总能抵御高浓度的变形虫。此外，根据基于生物学标准的评估，这些消毒程序的效率并不相同。此外，游泳池水的质量还取决于其源自来水的质量。因此，土壤变形虫的计数可作为评估游泳池水质量的额外生物学标志物。
