Lessire R, Juguelin H, Moreau P, Cassagne C
Arch Biochem Biophys. 1985 May 15;239(1):260-9. doi: 10.1016/0003-9861(85)90834-3.
The elongation of [1-14C]stearoyl-CoA by microsomes from etiolated leek seedlings, in the presence of malonyl-CoA and NADPH, has been studied at different substrate and enzyme concentrations. The HPTLC analysis of the whole reaction mixture, followed by the analysis of the label in the fatty acid methyl esters of long-chain acyl-CoAs, phosphatidylcholine (PC), and neutral lipids, showed that the acyl-CoA fraction contained most of the labeled very-long-chain fatty acids. The very-long-chain fatty acids were rapidly formed and released from the elongase(s) as acyl-CoAs. The label of long-chain acyl-CoAs increased for 20 min and then decreased, whereas it increased in PC. Labeled very-long-chain fatty acids appeared in the neutral lipid + free fatty acid fraction after a 20-min lag.
在不同底物和酶浓度下，研究了在丙二酸单酰辅酶A和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）存在的情况下，黄化韭菜幼苗微粒体对[1-¹⁴C]硬脂酰辅酶A的延长作用。对整个反应混合物进行高效薄层层析（HPTLC）分析，随后分析长链酰基辅酶A、磷脂酰胆碱（PC）和中性脂质的脂肪酸甲酯中的标记，结果表明酰基辅酶A部分含有大部分标记的超长链脂肪酸。超长链脂肪酸迅速形成并以酰基辅酶A的形式从延长酶中释放出来。长链酰基辅酶A的标记在20分钟内增加，然后减少，而在PC中则增加。经过20分钟的延迟后，标记的超长链脂肪酸出现在中性脂质+游离脂肪酸部分。
