与3个月大的兔子相比，1个月大的兔子眼外肌术后重塑时间更长。

One-Month-Old Rabbits Exhibit a Longer Postoperative Remodeling in Extraocular Muscles Compared to 3-Month-Old Rabbits.

作者信息

Lou Jiangtao, Huang Hailu, Peng Yiyi, Wang Chunxiao, Xu Meiping, Yu Xinping

机构信息

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China.

Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

出版信息

Invest Ophthalmol Vis Sci. 2025 Mar 3;66(3):12. doi: 10.1167/iovs.66.3.12.

DOI:10.1167/iovs.66.3.12

PMID:40042875

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11892532/

Abstract

PURPOSE

To explore whether there is a difference in postoperative extraocular muscle (EOM) remodeling between 1-month-old and 3-month-old rabbits.

METHODS

Recession (n = 16) and resection (n = 16) were performed on the right superior rectus (SR) muscles of 1-month-old and 3-month-old rabbits. SR tissues were harvested 1 and 4 weeks post-surgery (n = 4 for each group), and mid-belly sections were used to assess myosin heavy chain (MyHC) isoform expression (fast 2X, embryonic, and neonatal MyHCs), activated satellite cells (SCs), and centrally nucleated myofibers. Age-matched unoperated rabbits (n = 4) served as controls.

RESULTS

In 1-month-old rabbits, fast 2X-MyHC expression continuously decreased post-recession (all P < 0.01), and embryonic MyHC expression increased both post-recession and post-resection (all P < 0.01), except in the global layer (GL) post-resection. In 3-month-old rabbits, fast 2X-MyHC decreased at 1 week post-recession (in both layers P < 0.01) but returned to preoperative levels by 4 weeks (in both layers P = 1). Embryonic MyHC remained stable (P = 0.239) or increased (in the GL post-recession and in the orbital layer (OL) post-resection, both P < 0.001) at 1 week postoperatively, except in the GL post-resection. It had returned (in the GL post-recession, P = 0.952; in the GL post-resection, P = 0.574) or nearly returned (in the OL post-resection, P = 0.038) to preoperative levels in both layers by 4 weeks. Activated SCs in 1-month-old rabbits continuously increased post-surgery (all P < 0.05), except in the OL post-resection. In 3-month-old rabbits, SCs remained stable at 1 week but decreased by 4 weeks post-surgery (all P < 0.01), except in the OL post-resection. Centrally nucleated myofibers were more prevalent in 1-month-old rabbits by 4 weeks postoperatively.

CONCLUSIONS

One-month-old rabbits displayed longer postoperative remodeling and greater plasticity in EOMs than 3-month-old rabbits. The difference in postoperative remodeling may impact strabismus surgery outcomes in patients of different developmental stages.

摘要

目的

探讨1月龄和3月龄家兔术后眼外肌（EOM）重塑是否存在差异。

方法

对1月龄和3月龄家兔的右上直肌（SR）进行后徙术（n = 16）和切除术（n = 16）。术后1周和4周采集SR组织（每组n = 4），取肌腹中部切片评估肌球蛋白重链（MyHC）亚型表达（快肌2X、胚胎型和新生型MyHC）、活化卫星细胞（SCs）和中央核肌纤维。年龄匹配的未手术家兔（n = 4）作为对照。

结果

在1月龄家兔中，后徙术后快肌2X-MyHC表达持续下降（均P < 0.01），胚胎型MyHC表达在后徙术和切除术后均增加（均P < 0.01），切除术后球后层（GL）除外。在3月龄家兔中，后徙术后1周快肌2X-MyHC下降（两层均P < 0.01），但4周时恢复至术前水平（两层均P = 1）。术后1周，胚胎型MyHC保持稳定（P = 0.239）或增加（后徙术后GL和切除术后眶层（OL），均P < 0.001），切除术后GL除外。4周时，两层均已恢复（后徙术后GL，P = 0.952；切除术后GL，P = 0.574）或几乎恢复（切除术后OL，P = 0.038）至术前水平。1月龄家兔术后活化SCs持续增加（均P < 0.05），切除术后OL除外。在3月龄家兔中，SCs术后1周保持稳定，但4周时下降（均P < 0.01），切除术后OL除外。术后4周，1月龄家兔中央核肌纤维更为普遍。