Koops H P, Harms H
Arch Microbiol. 1985 Apr;141(3):214-8. doi: 10.1007/BF00408061.
DNA of 96 strains of the genera Nitrosomonas, Nitrosococcus, Nitrosospira, Nitrosolobus, and Nitrosovibrio was isolated and analysed spectrophotometrically. Percentages of guanine plus cytosine (G + C) content, genome sizes, and DNA-DNA homologies were determined. The results indicated the presence of eight Nitrosomonas species, three or four Nitrosococcus species, five Nitrosospira species, and two species of both Nitrosolobus and Nitrosovibrio. DNA homologies between strains of a separate species ranged from 56-100%. Average homologies between strains of different species were 33% in Nitrosococcus, 36% in Nitrosomonas, 37% in Nitrosolobus, 40% in Nitrosospira, and 42% in Nitrosovibrio. Average homologies between species of different genera were 33% and thus not significantly above the background value of 30% detected between DNA of ammonia-oxidizing bacteria and Escherichia coli. Genome sizes ranged from 1.90 - 2.74 X 10(9) dalton in Nitrosomonas, 2.09 - 2.37 X 10(9) dalton in Nitrosococcus, 1.87 - 2.15 X 10(9) dalton in Nitrosospira, 1.92 - 2.10 X 10(9) dalton in Nitrosolobus, and 1.91 - 2.15 X 10(9) dalton in Nitrosovibrio. Differences in genome sizes were in accordance with DNA homologies.
分离出96株亚硝化单胞菌属、亚硝化球菌属、亚硝化螺菌属、亚硝化叶菌属和亚硝化弧菌属细菌的DNA，并进行分光光度分析。测定了鸟嘌呤加胞嘧啶（G + C）含量百分比、基因组大小和DNA - DNA同源性。结果表明存在8种亚硝化单胞菌、3或4种亚硝化球菌、5种亚硝化螺菌，以及2种亚硝化叶菌和亚硝化弧菌。同一物种菌株之间的DNA同源性范围为56% - 100%。不同物种菌株之间的平均同源性在亚硝化球菌中为33%，在亚硝化单胞菌中为36%，在亚硝化叶菌中为37%，在亚硝化螺菌中为40%，在亚硝化弧菌中为42%。不同属物种之间的平均同源性为33%，因此并不显著高于氨氧化细菌与大肠杆菌DNA之间检测到的30%的背景值。基因组大小在亚硝化单胞菌中为1.90 - 2.74×10⁹道尔顿，在亚硝化球菌中为2.09 - 2.37×10⁹道尔顿，在亚硝化螺菌中为1.87 - 2.15×10⁹道尔顿，在亚硝化叶菌中为1.92 - 2.10×10⁹道尔顿，在亚硝化弧菌中为1.91 - 2.15×10⁹道尔顿。基因组大小的差异与DNA同源性一致。
