肝素抑制血管平滑肌细胞中的FoxO1/pFoxO1信号轴。
Heparin suppresses FoxO1/pFoxO1 signaling axis in vascular smooth muscle cells.
作者信息
Shokri Nafiseh, Elahimanesh Mohammad, Bakhshandeh Masoomeh, Najafi Mohammad
机构信息
Clinical Biochemistry Department, Faculty of Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Clinical Biochemistry Department, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Biochem Biophys Rep. 2025 Feb 19;41:101954. doi: 10.1016/j.bbrep.2025.101954. eCollection 2025 Mar.
BACKGROUND
The atherosclerosis process relates to the dysfunction of different cells in the plaque microenvironment. The vascular smooth muscle cells (VSMCs) play an important role in atherosclerosis. Since the FoxO family is reported to control cell growth, thus the aim of this study was to investigate the effects of Heparin, Betulinic acid, and Ibrutinib on the FoxO1/pFoxO1 axis in vascular smooth muscle cells.
METHODS
The VSMCs were cultured in DMEM-F12 medium and, were treated with Heparin (30IU), Betulinic acid (60 μM), and Ibrutinib (2 μM) in the 24 and 48-h periods. The FoxO1 gene expression level was identified using the RT-qPCR technique. The FoxO1 and pFoxO1 protein values were measured by the Western blot technique.
RESULTS
The FoxO1 gene expression levels were reduced significantly in the cellular groups treated with Heparin, Betulinic acid, and Ibrutinib (P < 0.05) in both periods. Moreover, the pFoxO1 and FoxO1 protein values were decreased by Heparin, and Betulinic acid in the VSMCs.
CONCLUSION
Heparin suppressed the FoxO1/pFoxO1 signaling axis in vascular smooth muscle cells (VSMCs). Furthermore, it modulated the effects of Betulinic acid and Ibrutinib.
背景
动脉粥样硬化过程与斑块微环境中不同细胞的功能障碍有关。血管平滑肌细胞（VSMC）在动脉粥样硬化中起重要作用。由于据报道FoxO家族可控制细胞生长，因此本研究的目的是探讨肝素、桦木酸和依鲁替尼对血管平滑肌细胞中FoxO1/pFoxO1轴的影响。
方法
将VSMC培养于DMEM-F12培养基中，并在24小时和48小时期间分别用肝素（30IU）、桦木酸（60μM）和依鲁替尼（2μM）处理。使用RT-qPCR技术鉴定FoxO1基因表达水平。通过蛋白质印迹技术测量FoxO1和pFoxO1蛋白值。
结果
在两个时间段内，用肝素、桦木酸和依鲁替尼处理的细胞组中FoxO1基因表达水平均显著降低（P<0.05）。此外，肝素和桦木酸降低了VSMC中pFoxO1和FoxO1蛋白值。
结论
肝素抑制血管平滑肌细胞（VSMC）中的FoxO1/pFoxO1信号轴。此外，它调节了桦木酸和依鲁替尼的作用。
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