Chudzińska-Skorupinska Jagoda, Wawrzyńczak Agata, Feliczak-Guzik Agnieszka
Department of Applied Chemistry, Faculty of Chemistry, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.
Micromachines (Basel). 2025 Jan 26;16(2):143. doi: 10.3390/mi16020143.
Recently, intensive research has been conducted on effective and simple systems for delivering active substances deep into the epidermis, e.g., for the treatment of skin inflammation. One possibility can be the use of soluble microneedles in which active compounds are encapsulated. This article describes the preparation of modern carriers, namely microneedles with encapsulated extracts of red beet or parsley leaves, that are rich in active substances with antioxidant and anti-inflammatory properties, specifically betanin and apigenin. The concentration of hyaluronic acid sodium salt, the method of preparing the solution, and the technique of the complete filling of molds were optimized. Plant extracts were obtained with sonication or maceration. In order to characterize the extracts obtained, several techniques were employed, such as UV-Vis, LC-MS, GC-MS, and FTIR-ATR. The analyses performed allowed for confirmation of the presence of selected active substances in the extracts. The most optimal solution of the microneedles' precursor turned out to be the one with a concentration of 10 wt.% of sodium hyaluronate, prepared by stirring and sonication. The most efficient extraction method for each plant was chosen, and the extracts were introduced into a solution of hyaluronic acid sodium salt. The resulting soluble microneedle patches can be used as an alternative to the traditional methods of delivering anti-inflammatory and antioxidant substances of plant origin.
最近，人们对将活性物质深入输送到表皮的有效且简单的系统进行了深入研究，例如用于治疗皮肤炎症。一种可能性是使用包封有活性化合物的可溶性微针。本文描述了现代载体的制备，即包封有红甜菜或欧芹叶提取物的微针，这些提取物富含具有抗氧化和抗炎特性的活性物质，特别是甜菜红素和芹菜素。对透明质酸钠盐的浓度、溶液制备方法以及模具完全填充技术进行了优化。通过超声处理或浸渍获得植物提取物。为了表征所获得的提取物，采用了几种技术，如紫外可见光谱、液相色谱 - 质谱联用、气相色谱 - 质谱联用和傅里叶变换红外光谱 - 衰减全反射法。所进行的分析证实了提取物中存在选定的活性物质。结果表明，微针前体的最佳溶液是通过搅拌和超声处理制备的浓度为10 wt.%的透明质酸钠溶液。选择了每种植物最有效的提取方法，并将提取物引入透明质酸钠盐溶液中。所得的可溶性微针贴片可作为传统方法输送植物源抗炎和抗氧化物质的替代方法。
