从多能干细胞衍生的II型样细胞分化为人肺泡I型上皮细胞的方案。
Protocol for the differentiation of human alveolar epithelial type I cells from pluripotent stem cell-derived type II-like cells.
作者信息
Burgess Claire L, Ayers Lauren J, Minakin Kasey, Alysandratos Konstantinos-Dionysios, Varelas Xaralabos, Kotton Darrell N
机构信息
Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center and Department of Medicine, Boston University Chobanian, Avedisian School of Medicine, Boston, MA 02118, USA.
Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA 02118, USA; The Pulmonary Center and Department of Medicine, Boston University Chobanian, Avedisian School of Medicine, Boston, MA 02118, USA.
出版信息
STAR Protoc. 2025 Mar 21;6(1):103667. doi: 10.1016/j.xpro.2025.103667. Epub 2025 Mar 5.
Pulmonary alveolar epithelial type I (AT1) cells have a flattened morphology to permit the diffusion of oxygen into the capillaries and historically have been difficult to isolate or maintain in culture. Here, we present a protocol for generating human alveolar type I-like cells (induced pluripotent stem cell-derived AT1s [iAT1s]) from induced pluripotent stem cell-derived alveolar epithelial type II cells (iAT2s) in vitro. We describe steps to plate iAT1s in either 3D or air-liquid interface cultures and to analyze or isolate iAT1s via flow cytometry. For complete details on the use and execution of this protocol, please refer to Burgess et al..
肺泡I型上皮（AT1）细胞形态扁平，有利于氧气扩散进入毛细血管，并且长期以来一直难以在体外分离或培养。在此，我们展示了一种从诱导多能干细胞衍生的肺泡II型上皮细胞（iAT2s）体外生成人肺泡I型样细胞（诱导多能干细胞衍生的AT1s [iAT1s]）的方案。我们描述了将iAT1s接种于三维或气液界面培养物中的步骤，以及通过流式细胞术分析或分离iAT1s的步骤。有关本方案使用和实施的完整细节，请参阅伯吉斯等人的文章。
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