基于重组Tc24抗原的磁免疫分析法快速高灵敏度检测克氏锥虫抗体：一项初步研究

Singampalli Kavya L, Zhan Bin, Mejia Rojelio, Lillehoj Peter B

Department of Bioengineering, Rice University.

Medical Scientist Training Program, Baylor College of Medicine.

J Infect Dis. 2025 Jul 11;231(6):e1114-e1118. doi: 10.1093/infdis/jiaf123.

The diagnosis of chronic Chagas disease is challenging due to the wide genetic diversity of Trypanosoma cruzi, the causative agent of Chagas disease, and low levels of parasitemia, resulting in low sensitivity and accuracy with existing diagnostics. We report a magneto-immunoassay that employs dually labeled magnetic beads incorporating a recombinant Tc24 antigen, which is homologous across multiple discrete typing units of T cruzi. In this pilot study, 102 serum samples from 7 endemic countries were tested by this magneto-immunoassay, revealing its ability to distinguish Chagas-positive from Chagas-negative cases more accurately and faster than a standard Tc24-based immunoassay.

由于恰加斯病的病原体克氏锥虫存在广泛的基因多样性，且寄生虫血症水平较低，导致现有诊断方法的敏感性和准确性较低，因此慢性恰加斯病的诊断具有挑战性。我们报告了一种磁免疫测定法，该方法采用了结合重组Tc24抗原的双标记磁珠，该抗原在克氏锥虫的多个离散型别单元中具有同源性。在这项初步研究中，通过这种磁免疫测定法对来自7个流行国家的102份血清样本进行了检测，结果表明，与基于Tc24的标准免疫测定法相比，该方法能够更准确、更快地区分恰加斯病阳性和阴性病例。