Bose S K, Mandal K, Chakrabarti B
Biochem Biophys Res Commun. 1985 May 16;128(3):1322-8. doi: 10.1016/0006-291x(85)91085-x.
Methylene blue- or riboflavin-sensitized photo-oxidation of alpha-crystallin caused a major change in the environment of thiol groups of the protein which could not be significantly prevented by inhibitors specific for active species of oxygen, 1O2, .OH, O.2, and H2O2. In contrast, the sensitizer N-formylkynurenine had no such effect. Sensitizer-induced change in the microenvironments of thiol groups was attributed to a conformational alteration, probably an unfolding of the protein.
亚甲蓝或核黄素敏化的α-晶状体蛋白光氧化作用导致该蛋白硫醇基团所处环境发生重大变化，而对氧的活性物种单线态氧、羟基自由基、超氧阴离子和过氧化氢具有特异性的抑制剂并不能显著阻止这种变化。相比之下，敏化剂N-甲酰犬尿氨酸则没有这种作用。敏化剂引起的硫醇基团微环境变化归因于构象改变，可能是蛋白质的去折叠。
